Reduced Life- and Healthspan in Mice Carrying a Mono-Allelic BubR1 MVA Mutation

Tobias Wijshake, Liviu A. Malureanu, Darren J Baker, Karthik B. Jeganathan, Bart van de Sluis, Jan Van Deursen

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Mosaic Variegated Aneuploidy (MVA) syndrome is a rare autosomal recessive disorder characterized by inaccurate chromosome segregation and high rates of near-diploid aneuploidy. Children with MVA syndrome die at an early age, are cancer prone, and have progeroid features like facial dysmorphisms, short stature, and cataracts. The majority of MVA cases are linked to mutations in BUBR1, a mitotic checkpoint gene required for proper chromosome segregation. Affected patients either have bi-allelic BUBR1 mutations, with one allele harboring a missense mutation and the other a nonsense mutation, or mono-allelic BUBR1 mutations combined with allelic variants that yield low amounts of wild-type BubR1 protein. Parents of MVA patients that carry single allele mutations have mild mitotic defects, but whether they are at risk for any of the pathologies associated with MVA syndrome is unknown. To address this, we engineered a mouse model for the nonsense mutation 2211insGTTA (referred to as GTTA) found in MVA patients with bi-allelic BUBR1 mutations. Here we report that both the median and maximum lifespans of the resulting BubR1+/GTTA mice are significantly reduced. Furthermore, BubR1+/GTTA mice develop several aging-related phenotypes at an accelerated rate, including cataract formation, lordokyphosis, skeletal muscle wasting, impaired exercise ability, and fat loss. BubR1+/GTTA mice develop mild aneuploidies and show enhanced growth of carcinogen-induced tumors. Collectively, these data demonstrate that the BUBR1 GTTA mutation compromises longevity and healthspan, raising the interesting possibility that mono-allelic changes in BUBR1 might contribute to differences in aging rates in the general population.

Original languageEnglish (US)
Article numbere1003138
JournalPLoS Genetics
Volume8
Issue number12
DOIs
StatePublished - Dec 2012

Fingerprint

aneuploidy
Aneuploidy
mutation
Mutation
mice
Chromosome Segregation
Nonsense Codon
nonsense mutation
Cataract
chromosome segregation
cataract
Alleles
M Phase Cell Cycle Checkpoints
Aptitude
chromosome
allele
Missense Mutation
alleles
Diploidy
Carcinogens

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Reduced Life- and Healthspan in Mice Carrying a Mono-Allelic BubR1 MVA Mutation. / Wijshake, Tobias; Malureanu, Liviu A.; Baker, Darren J; Jeganathan, Karthik B.; van de Sluis, Bart; Van Deursen, Jan.

In: PLoS Genetics, Vol. 8, No. 12, e1003138, 12.2012.

Research output: Contribution to journalArticle

Wijshake, Tobias ; Malureanu, Liviu A. ; Baker, Darren J ; Jeganathan, Karthik B. ; van de Sluis, Bart ; Van Deursen, Jan. / Reduced Life- and Healthspan in Mice Carrying a Mono-Allelic BubR1 MVA Mutation. In: PLoS Genetics. 2012 ; Vol. 8, No. 12.
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