Recycling of tumor necrosis factor-α receptor in MCF-7 cells

S. Vuk-Pavlovic, J. S. Kovach

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Kinetics of regulation of membrane receptors for tumor necrosis factor-α (TNF) in human breast adenocarcinoma MCF-7 cells was investigated. When MCF-7 cells were incubated with radioiodinated human recombinant TNF, they bound TNF specifically and accumulated it intracellularly. Preincubation of cells with native TNF up to 1 x 10-9 M for 12 h stimulated specific binding of TNF, indicating that concentrations of membrane receptors for TNF were regulated by the ligand. Accumulation of radioactivity in cells incubated with [125I]TNF proceeded at a constant rate for up to 24 h. Kinetics of binding and internalization of TNF were similar in the presence and absence of protein synthesis for at least 1 h, suggesting that the TNF receptor was either replenished from an intracellular pool of receptors or was recycled (reutilized) during the course of TNF internalization. Data were analyzed kinetically by fitting equations of compartmental models of ligand-cell interactions with and without the term for receptor-recycling. Fits were obtained only for the model with receptor recycling; absence of the term for receptor recycling resulted in physically impossible best-fit parameter values. Analysis of the best-fit parameters indicated that both internalization and recycling of the receptor were stimulated by the ligand.

Original languageEnglish (US)
Pages (from-to)2633-2640
Number of pages8
JournalFASEB Journal
Volume3
Issue number14
StatePublished - 1989

Fingerprint

tumor necrosis factors
Tumor Necrosis Factor Receptors
MCF-7 Cells
Recycling
recycling
Tumor Necrosis Factor-alpha
receptors
cells
Ligands
Membranes
Kinetics
Cell Communication
Radioactivity
Adenocarcinoma
Breast
kinetics
adenocarcinoma
breasts
protein synthesis
Proteins

Keywords

  • compartmental modeling
  • MCF-7 cells
  • receptor recycling
  • tumor necrosis factor-α receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Vuk-Pavlovic, S., & Kovach, J. S. (1989). Recycling of tumor necrosis factor-α receptor in MCF-7 cells. FASEB Journal, 3(14), 2633-2640.

Recycling of tumor necrosis factor-α receptor in MCF-7 cells. / Vuk-Pavlovic, S.; Kovach, J. S.

In: FASEB Journal, Vol. 3, No. 14, 1989, p. 2633-2640.

Research output: Contribution to journalArticle

Vuk-Pavlovic, S & Kovach, JS 1989, 'Recycling of tumor necrosis factor-α receptor in MCF-7 cells', FASEB Journal, vol. 3, no. 14, pp. 2633-2640.
Vuk-Pavlovic S, Kovach JS. Recycling of tumor necrosis factor-α receptor in MCF-7 cells. FASEB Journal. 1989;3(14):2633-2640.
Vuk-Pavlovic, S. ; Kovach, J. S. / Recycling of tumor necrosis factor-α receptor in MCF-7 cells. In: FASEB Journal. 1989 ; Vol. 3, No. 14. pp. 2633-2640.
@article{b9067ef3aca24c099f62f07f9b4cbecd,
title = "Recycling of tumor necrosis factor-α receptor in MCF-7 cells",
abstract = "Kinetics of regulation of membrane receptors for tumor necrosis factor-α (TNF) in human breast adenocarcinoma MCF-7 cells was investigated. When MCF-7 cells were incubated with radioiodinated human recombinant TNF, they bound TNF specifically and accumulated it intracellularly. Preincubation of cells with native TNF up to 1 x 10-9 M for 12 h stimulated specific binding of TNF, indicating that concentrations of membrane receptors for TNF were regulated by the ligand. Accumulation of radioactivity in cells incubated with [125I]TNF proceeded at a constant rate for up to 24 h. Kinetics of binding and internalization of TNF were similar in the presence and absence of protein synthesis for at least 1 h, suggesting that the TNF receptor was either replenished from an intracellular pool of receptors or was recycled (reutilized) during the course of TNF internalization. Data were analyzed kinetically by fitting equations of compartmental models of ligand-cell interactions with and without the term for receptor-recycling. Fits were obtained only for the model with receptor recycling; absence of the term for receptor recycling resulted in physically impossible best-fit parameter values. Analysis of the best-fit parameters indicated that both internalization and recycling of the receptor were stimulated by the ligand.",
keywords = "compartmental modeling, MCF-7 cells, receptor recycling, tumor necrosis factor-α receptors",
author = "S. Vuk-Pavlovic and Kovach, {J. S.}",
year = "1989",
language = "English (US)",
volume = "3",
pages = "2633--2640",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "14",

}

TY - JOUR

T1 - Recycling of tumor necrosis factor-α receptor in MCF-7 cells

AU - Vuk-Pavlovic, S.

AU - Kovach, J. S.

PY - 1989

Y1 - 1989

N2 - Kinetics of regulation of membrane receptors for tumor necrosis factor-α (TNF) in human breast adenocarcinoma MCF-7 cells was investigated. When MCF-7 cells were incubated with radioiodinated human recombinant TNF, they bound TNF specifically and accumulated it intracellularly. Preincubation of cells with native TNF up to 1 x 10-9 M for 12 h stimulated specific binding of TNF, indicating that concentrations of membrane receptors for TNF were regulated by the ligand. Accumulation of radioactivity in cells incubated with [125I]TNF proceeded at a constant rate for up to 24 h. Kinetics of binding and internalization of TNF were similar in the presence and absence of protein synthesis for at least 1 h, suggesting that the TNF receptor was either replenished from an intracellular pool of receptors or was recycled (reutilized) during the course of TNF internalization. Data were analyzed kinetically by fitting equations of compartmental models of ligand-cell interactions with and without the term for receptor-recycling. Fits were obtained only for the model with receptor recycling; absence of the term for receptor recycling resulted in physically impossible best-fit parameter values. Analysis of the best-fit parameters indicated that both internalization and recycling of the receptor were stimulated by the ligand.

AB - Kinetics of regulation of membrane receptors for tumor necrosis factor-α (TNF) in human breast adenocarcinoma MCF-7 cells was investigated. When MCF-7 cells were incubated with radioiodinated human recombinant TNF, they bound TNF specifically and accumulated it intracellularly. Preincubation of cells with native TNF up to 1 x 10-9 M for 12 h stimulated specific binding of TNF, indicating that concentrations of membrane receptors for TNF were regulated by the ligand. Accumulation of radioactivity in cells incubated with [125I]TNF proceeded at a constant rate for up to 24 h. Kinetics of binding and internalization of TNF were similar in the presence and absence of protein synthesis for at least 1 h, suggesting that the TNF receptor was either replenished from an intracellular pool of receptors or was recycled (reutilized) during the course of TNF internalization. Data were analyzed kinetically by fitting equations of compartmental models of ligand-cell interactions with and without the term for receptor-recycling. Fits were obtained only for the model with receptor recycling; absence of the term for receptor recycling resulted in physically impossible best-fit parameter values. Analysis of the best-fit parameters indicated that both internalization and recycling of the receptor were stimulated by the ligand.

KW - compartmental modeling

KW - MCF-7 cells

KW - receptor recycling

KW - tumor necrosis factor-α receptors

UR - http://www.scopus.com/inward/record.url?scp=0024805774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024805774&partnerID=8YFLogxK

M3 - Article

C2 - 2556313

AN - SCOPUS:0024805774

VL - 3

SP - 2633

EP - 2640

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 14

ER -