TY - JOUR
T1 - Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta 1
AU - Azmi, Ishara
AU - Davies, Brian
AU - Dimaano, Christian
AU - Payne, Johanna
AU - Eckert, Debra
AU - Babst, Markus
AU - Katzmann, David J.
PY - 2006/2/27
Y1 - 2006/2/27
N2 - In eukaryotes, the multivesicular body (MVB) sorting pathway plays an essential role in regulating cell surface protein composition, thereby impacting numerous cellular functions. Vps4, an ATPase associated with a variety of cellular activities, is required late in the MVB sorting reaction to dissociate the endosomal sorting complex required for transport (ESCRT), a requisite for proper function of this pathway. However, regulation of Vps4 function is not understood. We characterize Vta1 as a positive regulator of Vps4 both in vivo and in vitro. Vta1 promotes proper assembly of Vps4 and stimulates its ATPase activity through the conserved Vta1/SBP1/LIP5 region present in Vta1 homologues across evolution, including human SBP1 and Arabidopsis thaliana LIP5. These results suggest an evolutionarily conserved mechanism through which the disassembly of the ESCRT proteins, and thereby MVB sorting, is regulated by the Vta1/SBP1/LIP5 proteins.
AB - In eukaryotes, the multivesicular body (MVB) sorting pathway plays an essential role in regulating cell surface protein composition, thereby impacting numerous cellular functions. Vps4, an ATPase associated with a variety of cellular activities, is required late in the MVB sorting reaction to dissociate the endosomal sorting complex required for transport (ESCRT), a requisite for proper function of this pathway. However, regulation of Vps4 function is not understood. We characterize Vta1 as a positive regulator of Vps4 both in vivo and in vitro. Vta1 promotes proper assembly of Vps4 and stimulates its ATPase activity through the conserved Vta1/SBP1/LIP5 region present in Vta1 homologues across evolution, including human SBP1 and Arabidopsis thaliana LIP5. These results suggest an evolutionarily conserved mechanism through which the disassembly of the ESCRT proteins, and thereby MVB sorting, is regulated by the Vta1/SBP1/LIP5 proteins.
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U2 - 10.1083/jcb.200508166
DO - 10.1083/jcb.200508166
M3 - Article
C2 - 16505166
AN - SCOPUS:33644525938
SN - 0021-9525
VL - 172
SP - 705
EP - 717
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -