Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta 1

Ishara Azmi, Brian Davies, Christian Dimaano, Johanna Payne, Debra Eckert, Markus Babst, David J. Katzmann

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

In eukaryotes, the multivesicular body (MVB) sorting pathway plays an essential role in regulating cell surface protein composition, thereby impacting numerous cellular functions. Vps4, an ATPase associated with a variety of cellular activities, is required late in the MVB sorting reaction to dissociate the endosomal sorting complex required for transport (ESCRT), a requisite for proper function of this pathway. However, regulation of Vps4 function is not understood. We characterize Vta1 as a positive regulator of Vps4 both in vivo and in vitro. Vta1 promotes proper assembly of Vps4 and stimulates its ATPase activity through the conserved Vta1/SBP1/LIP5 region present in Vta1 homologues across evolution, including human SBP1 and Arabidopsis thaliana LIP5. These results suggest an evolutionarily conserved mechanism through which the disassembly of the ESCRT proteins, and thereby MVB sorting, is regulated by the Vta1/SBP1/LIP5 proteins.

Original languageEnglish (US)
Pages (from-to)705-717
Number of pages13
JournalJournal of Cell Biology
Volume172
Issue number5
DOIs
StatePublished - Feb 27 2006

ASJC Scopus subject areas

  • Cell Biology

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