Reconfigurable microfluidics combined with antibody microarrays for enhanced detection of T-cell secreted cytokines

Arnold Chen, Tam Vu, Gulnaz Stybayeva, Tingrui Pan, Alexander Revzin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cytokines are small proteins secreted by leukocytes in blood in response to infections, thus offering valuable diagnostic information. Given that the same cytokines may be produced by different leukocyte subsets in blood, it is beneficial to connect production of cytokines to specific cell types. In this paper, we describe integration of antibody (Ab) microarrays into a microfluidic device to enable enhanced cytokine detection. The Ab arrays contain spots specific to cell-surface antigens as well as anti-cytokine detection spots. Infusion of blood into a microfluidic device results in the capture of specific leukocytes (CD4 T-cells) and is followed by detection of secreted cytokines on the neighboring Ab spots using sandwich immunoassay. The enhancement of cytokine signal comes from leveraging the concept of reconfigurable microfluidics. A three layer polydimethylsiloxane microfluidic device is fabricated so as to contain six microchambers (1 mm × 1 mm × 30 μm) in the ceiling of the device. Once the T-cell capture is complete, the device is reconfigured by withdrawing liquid from the channel, causing the chambers to collapse onto Ab arrays and enclose cell/anti-cytokine spots within a 30 nl volume. In a set of proof-of-concept experiments, we demonstrate that ~90% pure CD4 T-cells can be captured inside the device and that signals for three important T-cell secreted cytokines, tissue necrosis factor-alpha, interferon-gamma, and interleukin-2, may be enhanced by 2 to 3 folds through the use of reconfigurable microfluidics.

Original languageEnglish (US)
Article number024105
JournalBiomicrofluidics
Volume7
Issue number2
DOIs
StatePublished - Apr 5 2013
Externally publishedYes

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Microfluidics
T-cells
Microarrays
antibodies
Antibodies
leukocytes
microfluidic devices
Lab-On-A-Chip Devices
Cytokines
blood
T-Lymphocytes
cells
interferon
interleukins
immunoassay
ceilings
necrosis
antigens
Leukocytes
infectious diseases

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Condensed Matter Physics
  • Materials Science(all)
  • Physical and Theoretical Chemistry

Cite this

Reconfigurable microfluidics combined with antibody microarrays for enhanced detection of T-cell secreted cytokines. / Chen, Arnold; Vu, Tam; Stybayeva, Gulnaz; Pan, Tingrui; Revzin, Alexander.

In: Biomicrofluidics, Vol. 7, No. 2, 024105, 05.04.2013.

Research output: Contribution to journalArticle

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