Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing

J. M. England, R. M. Rowan, O. W. Van Assendelft, B. S. Bull, W. Coulter, K. Fujimoto, W. Groner, A. Richardson-Jones, G. Klee, J. A. Koepke, S. M. Lewis, C. E. McLaren, N. K. Shinton, N. Tatsumi, R. L. Verwilghen

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Of the three ethylenediaminetetraacetic acid (EDTA) salts used for anticoagulation of blood specimens for hematologic testing, potassium salts are the most readily soluble. Tripotassium EDTA is dispensed as a liquid and thus causes a slight dilution of the specimen. This salt also has been shown to affect the red blood cell size more at increased concentrations and on storage than the dipotassium salt. Therefore, dipotassium EDTA is recommended as the anticoagulant of choice in specimen collection for blood cell counting and sizing. The amount of dipotassium EDTA used is 1.5-2.2 mg (3.7-5.4 μmol) per milliliter of blood.

Original languageEnglish (US)
Pages (from-to)371-372
Number of pages2
JournalAmerican Journal of Clinical Pathology
Volume100
Issue number4
StatePublished - 1993
Externally publishedYes

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Hematology
Edetic Acid
Blood Cells
Salts
Specimen Handling
Cell Size
Anticoagulants
Potassium
Erythrocytes

Keywords

  • Anticoagulant
  • Blood cell count
  • EDTA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

England, J. M., Rowan, R. M., Van Assendelft, O. W., Bull, B. S., Coulter, W., Fujimoto, K., ... Verwilghen, R. L. (1993). Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing. American Journal of Clinical Pathology, 100(4), 371-372.

Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing. / England, J. M.; Rowan, R. M.; Van Assendelft, O. W.; Bull, B. S.; Coulter, W.; Fujimoto, K.; Groner, W.; Richardson-Jones, A.; Klee, G.; Koepke, J. A.; Lewis, S. M.; McLaren, C. E.; Shinton, N. K.; Tatsumi, N.; Verwilghen, R. L.

In: American Journal of Clinical Pathology, Vol. 100, No. 4, 1993, p. 371-372.

Research output: Contribution to journalArticle

England, JM, Rowan, RM, Van Assendelft, OW, Bull, BS, Coulter, W, Fujimoto, K, Groner, W, Richardson-Jones, A, Klee, G, Koepke, JA, Lewis, SM, McLaren, CE, Shinton, NK, Tatsumi, N & Verwilghen, RL 1993, 'Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing', American Journal of Clinical Pathology, vol. 100, no. 4, pp. 371-372.
England, J. M. ; Rowan, R. M. ; Van Assendelft, O. W. ; Bull, B. S. ; Coulter, W. ; Fujimoto, K. ; Groner, W. ; Richardson-Jones, A. ; Klee, G. ; Koepke, J. A. ; Lewis, S. M. ; McLaren, C. E. ; Shinton, N. K. ; Tatsumi, N. ; Verwilghen, R. L. / Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing. In: American Journal of Clinical Pathology. 1993 ; Vol. 100, No. 4. pp. 371-372.
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