Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing

J. M. England, R. M. Rowan, O. W. Van Assendelft, B. S. Bull, W. Coulter, K. Fujimoto, W. Groner, A. Richardson-Jones, G. Klee, J. A. Koepke, S. M. Lewis, C. E. McLaren, N. K. Shinton, N. Tatsumi, R. L. Verwilghen

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

Of the three ethylenediaminetetraacetic acid (EDTA) salts used for anticoagulation of blood specimens for hematologic testing, potassium salts are the most readily soluble. Tripotassium EDTA is dispensed as a liquid and thus causes a slight dilution of the specimen. This salt also has been shown to affect the red blood cell size more at increased concentrations and on storage than the dipotassium salt. Therefore, dipotassium EDTA is recommended as the anticoagulant of choice in specimen collection for blood cell counting and sizing. The amount of dipotassium EDTA used is 1.5-2.2 mg (3.7-5.4 μmol) per milliliter of blood.

Original languageEnglish (US)
Pages (from-to)371-372
Number of pages2
JournalAmerican Journal of Clinical Pathology
Volume100
Issue number4
StatePublished - 1993
Externally publishedYes

Keywords

  • Anticoagulant
  • Blood cell count
  • EDTA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    England, J. M., Rowan, R. M., Van Assendelft, O. W., Bull, B. S., Coulter, W., Fujimoto, K., Groner, W., Richardson-Jones, A., Klee, G., Koepke, J. A., Lewis, S. M., McLaren, C. E., Shinton, N. K., Tatsumi, N., & Verwilghen, R. L. (1993). Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing. American Journal of Clinical Pathology, 100(4), 371-372.