Recommendations for Tissue Microarray Construction and Quality Assurance

Betty Glinsmann-Gibson, Lee Wisner, Melissa Stanton, Brandon Larsen, Lisa Rimsza, Alanna Maguire

Research output: Contribution to journalArticle

Abstract

Tissue microarrays (TMAs) are important tools to conserve precious tissue resources from increasingly smaller biopsies and to control experimental costs and variation across sample sets. The quality assurance assessment of TMA materials created at centralized biobanks has not been standardized. Herein, we outline 2 processes for the construction of TMAs ("recipient block" and "tape" methods) and the associated preconstruction quality control measures (pathology review, protein and RNA assessment, map creation, and storage conditions) developed by the AIDS Cancer Specimen Resource (ACSR) Network's Science and Technology Core. These steps provide a suggested framework for quality assessment that allows end-users, receiving materials from tissue banks, confidence in their experimental results.

Original languageEnglish (US)
JournalApplied Immunohistochemistry and Molecular Morphology
DOIs
StatePublished - Jan 1 2019

Keywords

  • immunohistochemistry
  • quality assurance
  • tissue microarray

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Medical Laboratory Technology

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