Recommendations for reference method for the packed cell volume (ICSH Standard 2001)

O. W. Van Assendelft, B. S. Bull, K. Fujimoto, W. Groner, B. Houwen, L. Van Hove, G. G. Klee, J. A. Koepke, S. M. Lewis, G. D'Onofrio, R. M. Rowan, N. Tatsumi, C. E. McLaren

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The manual, spun Packed Cell Volume (PCV, hematocrit) is a key measurement on which are based hematology instrument calibration, reference range determination and assignment of values to calibrators/controls. Until now, however, no true reference method for the PCV has existed. The International Council for Standardisation in Haematology (ICSH) which met in Montpelier, France in May 2001 has now recommended a reference method for the PCV. This reference PCV method is fully traceable to the ICSH reference method for haemoglobinometry. Using carefully defined capillary tubes, the reference PCV can be calculated as the ratio between the hemoglobin of whole blood and the hemoglobin of packed red blood cells with all plasma removed. The ICSH intends that this reference protocol be used primarily by manufacturers of capillary microhematocrit tubes, liquid calibrators and multichannel analyzers.

Original languageEnglish (US)
Pages (from-to)148-170
Number of pages23
JournalLaboratory Hematology
Volume7
Issue number3
StatePublished - 2001
Externally publishedYes

Fingerprint

Hematology
Cell Size
Standardization
Capillary tubes
Hemoglobinometry
Hemoglobins
Blood
Hematocrit
Calibration
France
Reference Values
Erythrocytes
Cells
Plasmas
Liquids

Keywords

  • Hematocrit
  • Packed cell volume (PCV)
  • Reference method

ASJC Scopus subject areas

  • Hematology

Cite this

Van Assendelft, O. W., Bull, B. S., Fujimoto, K., Groner, W., Houwen, B., Van Hove, L., ... McLaren, C. E. (2001). Recommendations for reference method for the packed cell volume (ICSH Standard 2001). Laboratory Hematology, 7(3), 148-170.

Recommendations for reference method for the packed cell volume (ICSH Standard 2001). / Van Assendelft, O. W.; Bull, B. S.; Fujimoto, K.; Groner, W.; Houwen, B.; Van Hove, L.; Klee, G. G.; Koepke, J. A.; Lewis, S. M.; D'Onofrio, G.; Rowan, R. M.; Tatsumi, N.; McLaren, C. E.

In: Laboratory Hematology, Vol. 7, No. 3, 2001, p. 148-170.

Research output: Contribution to journalArticle

Van Assendelft, OW, Bull, BS, Fujimoto, K, Groner, W, Houwen, B, Van Hove, L, Klee, GG, Koepke, JA, Lewis, SM, D'Onofrio, G, Rowan, RM, Tatsumi, N & McLaren, CE 2001, 'Recommendations for reference method for the packed cell volume (ICSH Standard 2001)', Laboratory Hematology, vol. 7, no. 3, pp. 148-170.
Van Assendelft OW, Bull BS, Fujimoto K, Groner W, Houwen B, Van Hove L et al. Recommendations for reference method for the packed cell volume (ICSH Standard 2001). Laboratory Hematology. 2001;7(3):148-170.
Van Assendelft, O. W. ; Bull, B. S. ; Fujimoto, K. ; Groner, W. ; Houwen, B. ; Van Hove, L. ; Klee, G. G. ; Koepke, J. A. ; Lewis, S. M. ; D'Onofrio, G. ; Rowan, R. M. ; Tatsumi, N. ; McLaren, C. E. / Recommendations for reference method for the packed cell volume (ICSH Standard 2001). In: Laboratory Hematology. 2001 ; Vol. 7, No. 3. pp. 148-170.
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