Receptor-mediated protein sorting to the vacuole in yeast

Roles for a Protein Kinase, a Lipid Kinase and GTP-Binding Proteins

Jeffrey H. Stack, Bruce F Horazdovsky, Scott D. Emr

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

In this review we the structural functional characteristics of the VPS (vacuolar protein sorting) products have provided into the regulatory interactions molecular mechanisms underlying sorting pathways in eukaryotic cells. Genetic selections in yeast have in the identification of 40 for the vesicle-mediated of proteins to the lysosome-like vacuole. Molecular of VPS gene products has revealed a number of biochemical activities involved in this process. Analogous to the mannose-6-phosphate in mammalian cells, the VPS10 a transmembrane sorting receptor for the yeast vacuolar hydrolase carboxypeptidase Y. The VPS15 and VPS34 genes encode components of a novel signal transduction complex essential for the delivery of soluble vacuolar hydrolases. VPS15 and VPS34 encode a serine/ threonine protein kinase a phosphatidylinositol 3-kinase, respectively, that interact at the cytoplasmic face of an intracellular membrane compartment, most likely corresponding to the late Golgi. other VPS gene products have homologues that are involved in membrane trafficking pathways: The VPS1 and VPS21 genes encode GTPases of the dynamin rab families, respectively, and the products of the VPS33, VPS45, and PEP12/VPS6 are homologues of proteins involved in regulated synaptic vesicle exocytosis. The VPSgene products constitute components of a molecular apparatus responsible for the recognition, packaging, and vesicular transport of proteins to the vacuole in yeast.

Original languageEnglish (US)
Pages (from-to)1-33
Number of pages33
JournalAnnual Review of Cell and Developmental Biology
Volume11
StatePublished - 1995
Externally publishedYes

Fingerprint

Protein Transport
Vacuoles
GTP-Binding Proteins
Protein Kinases
Phosphotransferases
Yeasts
Lipids
Hydrolases
Vesicular Transport Proteins
Proteins
rab GTP-Binding Proteins
Cathepsin A
Phosphatidylinositol 3-Kinase
Gene Components
Dynamins
Intracellular Membranes
Synaptic Vesicles
Genetic Selection
Protein-Serine-Threonine Kinases
Exocytosis

Keywords

  • Lysosome
  • Mannose-6-phosphate receptor
  • Phosphatidylinositol 3-kinase
  • VPS genes

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Cite this

Receptor-mediated protein sorting to the vacuole in yeast : Roles for a Protein Kinase, a Lipid Kinase and GTP-Binding Proteins. / Stack, Jeffrey H.; Horazdovsky, Bruce F; Emr, Scott D.

In: Annual Review of Cell and Developmental Biology, Vol. 11, 1995, p. 1-33.

Research output: Contribution to journalArticle

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