Recent advances in the isolation of liver cells

Gianfranco Alpini, John O. Phillips, Benjamin Vroman, Nicholas F La Russo

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

The development of new and refined separation techniques-including FACS, FFE, CFE and isopyknic gradients-has had a profound impact on the ability of investigators to isolate specific cell types from the liver. Although some of these techniques, such as FFE, may be of limited preparative value, they are nonetheless important analytical tools that detect subtle differences among cell subpopulations. The isolation of highly purified preparations of liver cells in large yields requires the use of more conventional purification methods such as CFE and isopyknic centrifugation. Immunological approaches represent a key development for the isolation of specific liver cell types, especially when they are used in combination with other techniques. Excellent, reliable and relatively simple techniques now exist to isolate highly purified preparations of hepatocytes, cholangiocytes, KCs, SCs, FSC, myofibroblasts and pit cells. Additional work is necessary to refine techniques for the isolation of dendritic cells and lymphocytes.

Original languageEnglish (US)
Pages (from-to)494-514
Number of pages21
JournalHepatology
Volume20
Issue number2
DOIs
StatePublished - Aug 1994

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Cell Separation
Liver
Myofibroblasts
Centrifugation
Dendritic Cells
Hepatocytes
Research Personnel
Lymphocytes

ASJC Scopus subject areas

  • Hepatology

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Recent advances in the isolation of liver cells. / Alpini, Gianfranco; Phillips, John O.; Vroman, Benjamin; La Russo, Nicholas F.

In: Hepatology, Vol. 20, No. 2, 08.1994, p. 494-514.

Research output: Contribution to journalArticle

Alpini, Gianfranco ; Phillips, John O. ; Vroman, Benjamin ; La Russo, Nicholas F. / Recent advances in the isolation of liver cells. In: Hepatology. 1994 ; Vol. 20, No. 2. pp. 494-514.
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