Reassessment of the Transport Mechanism of the Human Zinc Transporter SLC39A2

The human zinc transporter SLC39A2, also known as ZIP2, was shown to mediate zinc transport that could be inhibited at pH <7.0 and stimulated by HCO₃ ^-, suggesting a Zn²⁺/HCO₃ ^- cotransport mechanism [Gaither, L. A., and Eide, D. J. (2000) J. Biol. Chem. 275, 5560-5564]. In contrast, recent experiments in our laboratory indicated that the functional activity of ZIP2 increases at acidic pH [Franz, M. C., et al. (2014) J. Biomol. Screening 19, 909-916]. The study presented here was therefore designed to reexamine the findings about the pH dependence and to extend the functional characterization of ZIP2. Our current results show that ZIP2-mediated transport is modulated by extracellular pH but independent of the H⁺ driving force. Also, in our experiments, ZIP2-mediated transport is not modulated by extracellular HCO₃ ^-. Moreover, a high extracellular [K⁺], which induces depolarization, inhibited ZIP2-mediated transport, indicating that the transport mechanism is voltage-dependent. We also show that ZIP2 mediates the uptake of Cd²⁺ (K_m ∼ 1.57 μM) in a pH-dependent manner (K_H+ ∼ 66 nM). Cd²⁺ transport is inhibited by extracellular [Zn²⁺] (IC₅₀ ∼ 0.32 μM), [Cu²⁺] (IC₅₀ ∼ 1.81 μM), and to a lesser extent [Co²⁺], but not by [Mn²⁺] or [Ba²⁺]. Fe²⁺ is not transported by ZIP2. Accordingly, the substrate selectivity of ZIP2 decreases in the following order: Zn²⁺ > Cd²⁺ ≥ Cu²⁺ > Co²⁺. Altogether, we propose that ZIP2 is a facilitated divalent metal ion transporter that can be modulated by extracellular pH and membrane potential. Given that ZIP2 expression has been reported in acidic environments [Desouki, M. M., et al. (2007) Mol. Cancer 6, 37; Inoue, Y., et al. (2014) J. Biol. Chem. 289, 21451-21462; Tao, Y. T., et al. (2013) Mol. Biol. Rep. 40, 4979-4984], we suggest that the herein described H⁺-mediated regulatory mechanism might be important for determining the velocity and direction of the transport process.