Rearrangement in the coding region of the MYCN gene in a subset of amplicons in a case of neuroblastoma with MYCN amplification

Beiyun Chen, Suresh C. Jhanwar, Marc Ladanyi

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The MYCN gene is often amplified but rarely rearranged in neuroblastoma. We report, for the first time, a rearrangement within the MYCN coding region in a metastatic neuroblastoma in a 3-year-old boy with MYCN amplification in his primary tumor. The rearrangement occurred 46 nucleotides downstream from the ATG codon in exon 2 of MYCN. The amplification level of the rearranged copies of the MYCN gene was lower than that of the unrearranged copies of MYCN. These results indicate that the rearrangement occurred after initial MYCN gene amplification. Monochromosomal somatic cell hybrid mapping of the novel region fused to exon 2 of MYCN localized it to chromosome 2, suggesting that this rearrangement resulted from an interstitial deletion, presumably within the MYCN amplicon itself.

Original languageEnglish (US)
Pages (from-to)100-104
Number of pages5
JournalDiagnostic Molecular Pathology
Volume10
Issue number2
DOIs
StatePublished - Jun 21 2001

Keywords

  • Amplification
  • MYCN gene
  • Neuroblastoma
  • Rearrangement

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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