Real-Time PCR Assay for Detection of Kingella kingae in Children

Theresa Madigan, Scott A. Cunningham, Poornima Ramanan, Micah M. Bhatti, Robin Patel

Research output: Contribution to journalArticle

Abstract

Background Kingella kingae is a known cause of osteoarticular infections in children younger than 4 years of age, but it is not always recoverable in culture. Molecular methods are increasingly used for diagnosis. Methods To facilitate diagnosis of K. kingae septic arthritis, we developed a real-time polymerase chain reaction (PCR) assay for the detection of K. kingae that targets the repeat-in-toxin gene ( rtxB ). Results We present three pediatric patients with K. kingae septic arthritis at our institution who were diagnosed using the real-time PCR assay. All underwent arthrotomy with irrigation and debridement and were symptom-free after 3 weeks of therapy with β-lactam antibiotics. Cultures of synovial fluid or tissue grew K. kingae in two of three; K. kingae real-time PCR was positive in all three patients. In addition, 11 cases of K. kingae osteoarticular infection were diagnosed through Mayo Medical Laboratories using this assay. The limit of detection of the real-time PCR assay was 73.7 colony-forming unit (CFU)/µL for tissue and 1.3 CFU/µL for synovial fluid. Conclusions PCR-based detection methods are faster and more sensitive than conventional culture-based methods for the diagnosis of K. kingae osteoarticular infections in children.

Original languageEnglish (US)
JournalJournal of Pediatric Infectious Diseases
DOIs
StateAccepted/In press - Apr 11 2018

Fingerprint

Kingella kingae
Real-Time Polymerase Chain Reaction
Infectious Arthritis
Synovial Fluid
Stem Cells
Infection
Lactams
Debridement
Limit of Detection
Pediatrics
Anti-Bacterial Agents

Keywords

  • children
  • Kingella kingae
  • real-time PCR

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Infectious Diseases

Cite this

Real-Time PCR Assay for Detection of Kingella kingae in Children . / Madigan, Theresa; Cunningham, Scott A.; Ramanan, Poornima; Bhatti, Micah M.; Patel, Robin.

In: Journal of Pediatric Infectious Diseases, 11.04.2018.

Research output: Contribution to journalArticle

Madigan, Theresa ; Cunningham, Scott A. ; Ramanan, Poornima ; Bhatti, Micah M. ; Patel, Robin. / Real-Time PCR Assay for Detection of Kingella kingae in Children . In: Journal of Pediatric Infectious Diseases. 2018.
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