TY - JOUR
T1 - Real-time monitoring and analysis of zebrafish electrocardiogram with anomaly detection
AU - Lenning, Michael
AU - Fortunato, Joseph
AU - Le, Tai
AU - Clark, Isaac
AU - Sherpa, Ang
AU - Yi, Soyeon
AU - Hofsteen, Peter
AU - Thamilarasu, Geethapriya
AU - Yang, Jingchun
AU - Xu, Xiaolei
AU - Han, Huy Dung
AU - Hsiai, Tzung K.
AU - Cao, Hung
N1 - Publisher Copyright:
© 2017 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2018/1
Y1 - 2018/1
N2 - Heart disease is the leading cause of mortality in the U.S. with approximately 610,000 people dying every year. Effective therapies for many cardiac diseases are lacking, largely due to an incomplete understanding of their genetic basis and underlying molecular mechanisms. Zebrafish (Danio rerio) are an excellent model system for studying heart disease as they enable a forward genetic approach to tackle this unmet medical need. In recent years, our team has been employing electrocardiogram (ECG) as an efficient tool to study the zebrafish heart along with conventional approaches, such as immunohistochemistry, DNA and protein analyses. We have overcome various challenges in the small size and aquatic environment of zebrafish in order to obtain ECG signals with favorable signal-to-noise ratio (SNR), and high spatial and temporal resolution. In this paper, we highlight our recent efforts in zebrafish ECG acquisition with a cost-effective simplified microelectrode array (MEA) membrane providing multi-channel recording, a novel multi-chamber apparatus for simultaneous screening, and a LabVIEW program to facilitate recording and processing. We also demonstrate the use of machine learning-based programs to recognize specific ECG patterns, yielding promising results with our current limited amount of zebrafish data. Our solutions hold promise to carry out numerous studies of heart diseases, drug screening, stem cell-based therapy validation, and regenerative medicine.
AB - Heart disease is the leading cause of mortality in the U.S. with approximately 610,000 people dying every year. Effective therapies for many cardiac diseases are lacking, largely due to an incomplete understanding of their genetic basis and underlying molecular mechanisms. Zebrafish (Danio rerio) are an excellent model system for studying heart disease as they enable a forward genetic approach to tackle this unmet medical need. In recent years, our team has been employing electrocardiogram (ECG) as an efficient tool to study the zebrafish heart along with conventional approaches, such as immunohistochemistry, DNA and protein analyses. We have overcome various challenges in the small size and aquatic environment of zebrafish in order to obtain ECG signals with favorable signal-to-noise ratio (SNR), and high spatial and temporal resolution. In this paper, we highlight our recent efforts in zebrafish ECG acquisition with a cost-effective simplified microelectrode array (MEA) membrane providing multi-channel recording, a novel multi-chamber apparatus for simultaneous screening, and a LabVIEW program to facilitate recording and processing. We also demonstrate the use of machine learning-based programs to recognize specific ECG patterns, yielding promising results with our current limited amount of zebrafish data. Our solutions hold promise to carry out numerous studies of heart diseases, drug screening, stem cell-based therapy validation, and regenerative medicine.
KW - ECG pattern recognition
KW - Electrocardiogram (ECG)
KW - Heart diseases
KW - Machine learning
KW - Phenotype screening
KW - Real-time monitoring
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85039918245&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85039918245&partnerID=8YFLogxK
U2 - 10.3390/s18010061
DO - 10.3390/s18010061
M3 - Article
C2 - 29283402
AN - SCOPUS:85039918245
SN - 1424-8220
VL - 18
JO - Sensors (Switzerland)
JF - Sensors (Switzerland)
IS - 1
M1 - 61
ER -