Reactive oxygen species detection in senescent cells

Stella Victorelli, Joao Passos

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Cumulative evidence suggests that cellular senescence plays a variety of important physiological roles, including tumor suppression, embryonic development and ageing. Senescent cells are characterized by increased production of reactive oxygen species (ROS), mostly produced by dysfunctional mitochondria. Both intracellular and extracellular ROS have been shown to contribute to the induction of senescence. ROS have also been shown to act as signaling molecules during senescence, stabilizing the cell-cycle arrest. In this chapter, we present a detailed description of protocols that allow us to characterize intracellular and extracellular ROS in live senescent cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages21-29
Number of pages9
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1896
ISSN (Print)1064-3745

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Keywords

  • Amplex Red
  • DHE
  • DHR123
  • MitoSOX™
  • ROS
  • ROS indicator dyes
  • Senescence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Victorelli, S., & Passos, J. (2019). Reactive oxygen species detection in senescent cells. In Methods in Molecular Biology (pp. 21-29). (Methods in Molecular Biology; Vol. 1896). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8931-7_3