Reactions of 1-N6-ethenoadenosine nucleotides with myosin subfragment 1 and acto-subfragment 1 of skeletal and smooth muscle

Steven Rosenfeld, E. W. Taylor

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The fluorescent nucleotides εADP and εATP were used to study the binding and hydrolysis mechanisms of subfragment 1 (S-1) and acto-subfragment 1 from striated and smooth muscle. The quenching of the enhanced fluorescence emission of bound nucleotide by acrylamide analyzed either by the Stern-Volmer method or by fluorescence lifetime measurements showed the presence of two bound nucleotide states for 1-N6-ethenoadenosine triphosphate (εATP), 1-N6-ethenoadenosine diphosphate (εADP), and εADP-vanadate complexes with S-1. The equilibrium constant relating the two bound nucleotide states was close to unity. Transient kinetic studies showed two first-order transitions with rate constants of approximately 500 and 100 s-1 for both εATP and εADP and striated muscle S-1 and 300 and 30 s-1, respectively, for smooth muscle S-1. The hydrolysis of [γ-32P]εATP yielded a transient phase of small amplitude (less than 0.2 mol/site) with a rate constant of 5-10 s-1. Consequently, the hydrolysis of the substrate is a step in the mechanism which is distinct from the two conformational changes induced by the binding of εATP. An essentially symmetric reaction mechanism is proposed in which two structural changes accompany substrate binding and the reversal of these steps occurs in product release. εATP dissociates acto-S-1 as effectively as ATP. For smooth muscle acto-S-1, dissociation proceeds in two steps, each accompanied by enhancement of fluorescence emission. A symmetric reaction scheme is proposed for the acto-S-1 εATPase cycle. The very similar kinetic properties of the reactions of εATP and ATP with S-1 and acto-S-1 suggest that two ATP intermediate states also occur in the ATPase reaction mechanism.

Original languageEnglish (US)
Pages (from-to)11920-11929
Number of pages10
JournalJournal of Biological Chemistry
Volume259
Issue number19
StatePublished - Jan 1 1984
Externally publishedYes

Fingerprint

pioglitazone
Myosin Subfragments
Smooth Muscle
Muscle
Skeletal Muscle
Nucleotides
Adenosine Triphosphate
Adenosine Diphosphate
Hydrolysis
Striated Muscle
Fluorescence
Adenosine Triphosphatases
Ethenoadenosine Triphosphate
Rate constants
Kinetics
Vanadates
Acrylamide

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Reactions of 1-N6-ethenoadenosine nucleotides with myosin subfragment 1 and acto-subfragment 1 of skeletal and smooth muscle. / Rosenfeld, Steven; Taylor, E. W.

In: Journal of Biological Chemistry, Vol. 259, No. 19, 01.01.1984, p. 11920-11929.

Research output: Contribution to journalArticle

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