Re-infused autologous graft natural killer cells correlates with absolute lymphocyte count recovery after autologous stem cell transplantation

Luis F. Porrata, Dennis A. Gastineau, Douglas Padley, Kevin Bundy, Svetomir N. Markovic

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Early absolute lymphocyte count (ALC) has been reported to be a powerful prognostic indicator of survival after autologous stem cell transplantation (ASCT). One possible source affecting ALC recovery includes the re-infused autologous graft lymphocytes (AGL). To assess if the re-infused AGL correlate with ALC recovery post-ASCT, we conducted a pilot study to identify which of the re-infused AGL subsets is most associated with day 15 ALC recovery in three patients with multiple myeloma and four patients with non-Hodgkin's lymphoma. Using the Spearman rank correlation coefficient analysis (r), we compared absolute numbers of CD3, CD4, CD8, CD19, and CD16+/CD56+ cells/kg of body weight from the apheresis product with ALC (cells/μl) at day 15 post-ASCT. The main lymphocyte subsets identified in the apheresis product were T cells and NK cells. There was no strong correlation between T or B cells from the apheresis product compared with the ALC at day 15 post-ASCT (CD3, r = 0.21; CD4, r = 0.32; CD8, r = 0.39; and CD19, r = 0.14). However, there was good correlation between NK cells from the apheresis product compared with ALC at day 15 post-ASCT (CD16+/CD56+/CD3-, r = 0.77). These data provide preliminary evidence that the number of re-infused autologous graft NK cells in the apheresis product significantly affect ALC recovery early post-ASCT. However, given the small sample size, our results are primarily hypothesis generating and subject of further research.

Original languageEnglish (US)
Pages (from-to)997-1000
Number of pages4
JournalLeukemia and Lymphoma
Volume44
Issue number6
DOIs
StatePublished - Jun 1 2003

Keywords

  • Absolute lymphocyte count
  • Autologous graft lymphocyte subsets
  • Autologous stem cell transplantation
  • Natural killer cells

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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