Rat sciatic nerve repair with a poly-lactic-co-glycolic acid scaffold and nerve growth factor releasing microspheres

Ralph De Boer, Andrew M. Knight, Andreas Borntraeger, Marie Noëlle Hébert-Blouin, Robert J. Spinner, Martijn J A Malessy, Michael J Yaszemski, Anthony John Windebank

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The effect of microsphere delivered Nerve Growth Factor (NGF) in a poly-lactic-co-glycolic-acid (PLGA) 85/15 nerve conduit bridging a 10mm rat sciatic nerve gap was assessed, comparing nine groups (n = 6): PLGA conduits filled with saline, saline and NGF, saline with blank microspheres; four different NGF microspheres (5, 20, 50, and 100 mg/ml); an autologous graft and sciatic nerve gap. Histomorphometry, retrograde tracing, electrophysiology, and functional outcomes were evaluated up to 16 weeks. The autologous graft showed the largest fascicular area (0.65 mm2) and had a significantly greater number of myelinated fibers (P < 0.0001). Electrophysiology showed Compound Muscle Action Potential (CMAP) recordings for the autologous graft returning at 6 weeks after nerve transection, reaching their highest amplitude of 3.6 mV at endpoint. No significant differences were found in functional evaluation between groups or between conduits with microspheres and the saline filled conduit. A PLGA 85/15 nerve conduit is capable of sustaining nerve regeneration. The microsphere delivery system does not interfere with regeneration.

Original languageEnglish (US)
Pages (from-to)293-302
Number of pages10
JournalMicrosurgery
Volume31
Issue number4
DOIs
StatePublished - May 2011

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Nerve Growth Factor
Sciatic Nerve
Microspheres
Electrophysiology
Transplants
Nerve Regeneration
Action Potentials
Regeneration
polylactic acid-polyglycolic acid copolymer
Muscles

ASJC Scopus subject areas

  • Surgery

Cite this

De Boer, R., Knight, A. M., Borntraeger, A., Hébert-Blouin, M. N., Spinner, R. J., Malessy, M. J. A., ... Windebank, A. J. (2011). Rat sciatic nerve repair with a poly-lactic-co-glycolic acid scaffold and nerve growth factor releasing microspheres. Microsurgery, 31(4), 293-302. https://doi.org/10.1002/micr.20869

Rat sciatic nerve repair with a poly-lactic-co-glycolic acid scaffold and nerve growth factor releasing microspheres. / De Boer, Ralph; Knight, Andrew M.; Borntraeger, Andreas; Hébert-Blouin, Marie Noëlle; Spinner, Robert J.; Malessy, Martijn J A; Yaszemski, Michael J; Windebank, Anthony John.

In: Microsurgery, Vol. 31, No. 4, 05.2011, p. 293-302.

Research output: Contribution to journalArticle

De Boer R, Knight AM, Borntraeger A, Hébert-Blouin MN, Spinner RJ, Malessy MJA et al. Rat sciatic nerve repair with a poly-lactic-co-glycolic acid scaffold and nerve growth factor releasing microspheres. Microsurgery. 2011 May;31(4):293-302. https://doi.org/10.1002/micr.20869
De Boer, Ralph ; Knight, Andrew M. ; Borntraeger, Andreas ; Hébert-Blouin, Marie Noëlle ; Spinner, Robert J. ; Malessy, Martijn J A ; Yaszemski, Michael J ; Windebank, Anthony John. / Rat sciatic nerve repair with a poly-lactic-co-glycolic acid scaffold and nerve growth factor releasing microspheres. In: Microsurgery. 2011 ; Vol. 31, No. 4. pp. 293-302.
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