Rat astrocytes and Schwann cells in culture synthesize nerve growth factor-like neurite-promoting factors

Jose G. Assouline, Peter Bosch, Ramon Lim, In Sook Kim, Robert Jensen, Nicholas J. Pantazis

Research output: Contribution to journalArticle

148 Scopus citations

Abstract

Neurite-promoting activity in feeding medium conditioned by rat astrocytes and Schwann cells in culture was examined. The conditioned medium (CM) from both types of glial cultures stimulated extensive neurite outgrowth from embryonic chick dorsal root ganglia (DRG) as well as pheochromocytoma (PC12) cells. Both the DRG and PC12 cells also produce neurite outgrowth in the presence of nerve growth factor (NGF). With the DRG, the neurite growth rates observed with the glial cell CM were identical to growth rates seen with NGF. Although anti-NGF antibody did not inhibit the neurite outgrowth produced by either of the glial CM, a nerve growth factor radioreceptor assay did detect an NGF-like molecule in both CM. Since the extensive neurite outgrowth stimulated by the glial CM was not mimicked by pure laminin alone, we conclude that the glial neurite promoting factors are distinct from laminin.

Original languageEnglish (US)
Pages (from-to)103-118
Number of pages16
JournalDevelopmental Brain Research
Volume31
Issue number1
DOIs
StatePublished - Jan 1987

Keywords

  • Astrocyte
  • Nerve growth factor
  • Neurite-promoting factor
  • Schwann cell

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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