Rare SNP rs12731181 in the miR-590-3p target site of the prostaglandin F receptor gene confers risk for essential hypertension in the han Chinese population

Bing Xiao, Shui Ming Gu, Mulin Jun Li, Jun Li, Bo Tao, Yuanyang Wang, Yan Wang, Shengkai Zuo, Yujun Shen, Yu Yu, Di Chen, Guilin Chen, Deping Kong, Juan Tang, Qian Liu, Dong Rui Chen, Yong Liu, Sara Alberti, Melania Dovizio, Raffaele Landolfi & 8 others Luciana Mucci, Pei Zhi Miao, Pingjin Gao, Ding Liang Zhu, Junwen Wang, Bin Li, Paola Patrignani, Ying Yu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective-To investigate whether rs12731181 (A→G) interrupted miR-590-3p-mediated suppression of the prostaglandin F receptor (FP) and whether it is associated with essential hypertension in the Chinese population. Approach and Results-We found that miR-590-3p regulates human FP gene expression by binding to its 3′-untranslated region. rs12731181 (A→G) altered the binding affinity between miR-590-3p and its FP 3′-untranslated region target, thus reducing the suppression of FP expression, which, in turn, enhanced FP receptor-mediated contractility of vascular smooth muscle cells. Overexpression of FP augmented vascular tone and elevated blood pressure in mice. An association study was performed to analyze the relationship between the FP gene and essential hypertension in the Han Chinese population. The results indicated that the rs12731181 G allele was associated with susceptibility to essential hypertension. Carriers of the AG genotype exhibited significantly higher blood pressure than those of the AA genotype. FP gene expression was significantly higher in human peripheral leukocytes from individuals with the AG genotype than that in leukocytes from individuals with the AA genotype. Conclusions-rs12731181 in the seed region of the miR-590-3p target site is associated with increased risk of essential hypertension and represents a new paradigm for FP involvement in blood pressure regulation.

Original languageEnglish (US)
Pages (from-to)1687-1695
Number of pages9
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume35
Issue number7
DOIs
StatePublished - Jul 27 2015
Externally publishedYes

Fingerprint

Single Nucleotide Polymorphism
Genotype
3' Untranslated Regions
Population
Genes
Leukocytes
Blood Pressure
Gene Expression
Vascular Smooth Muscle
Smooth Muscle Myocytes
Blood Vessels
Seeds
Alleles
Hypertension
prostaglandin F2alpha receptor
Essential Hypertension

Keywords

  • essential
  • hypertension
  • microRNAs
  • polymorphism
  • prostaglandin F<inf>2α</inf> receptor
  • single nucleotide

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Medicine(all)

Cite this

Rare SNP rs12731181 in the miR-590-3p target site of the prostaglandin F receptor gene confers risk for essential hypertension in the han Chinese population. / Xiao, Bing; Gu, Shui Ming; Li, Mulin Jun; Li, Jun; Tao, Bo; Wang, Yuanyang; Wang, Yan; Zuo, Shengkai; Shen, Yujun; Yu, Yu; Chen, Di; Chen, Guilin; Kong, Deping; Tang, Juan; Liu, Qian; Chen, Dong Rui; Liu, Yong; Alberti, Sara; Dovizio, Melania; Landolfi, Raffaele; Mucci, Luciana; Miao, Pei Zhi; Gao, Pingjin; Zhu, Ding Liang; Wang, Junwen; Li, Bin; Patrignani, Paola; Yu, Ying.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 35, No. 7, 27.07.2015, p. 1687-1695.

Research output: Contribution to journalArticle

Xiao, B, Gu, SM, Li, MJ, Li, J, Tao, B, Wang, Y, Wang, Y, Zuo, S, Shen, Y, Yu, Y, Chen, D, Chen, G, Kong, D, Tang, J, Liu, Q, Chen, DR, Liu, Y, Alberti, S, Dovizio, M, Landolfi, R, Mucci, L, Miao, PZ, Gao, P, Zhu, DL, Wang, J, Li, B, Patrignani, P & Yu, Y 2015, 'Rare SNP rs12731181 in the miR-590-3p target site of the prostaglandin F receptor gene confers risk for essential hypertension in the han Chinese population', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 35, no. 7, pp. 1687-1695. https://doi.org/10.1161/ATVBAHA.115.305445
Xiao, Bing ; Gu, Shui Ming ; Li, Mulin Jun ; Li, Jun ; Tao, Bo ; Wang, Yuanyang ; Wang, Yan ; Zuo, Shengkai ; Shen, Yujun ; Yu, Yu ; Chen, Di ; Chen, Guilin ; Kong, Deping ; Tang, Juan ; Liu, Qian ; Chen, Dong Rui ; Liu, Yong ; Alberti, Sara ; Dovizio, Melania ; Landolfi, Raffaele ; Mucci, Luciana ; Miao, Pei Zhi ; Gao, Pingjin ; Zhu, Ding Liang ; Wang, Junwen ; Li, Bin ; Patrignani, Paola ; Yu, Ying. / Rare SNP rs12731181 in the miR-590-3p target site of the prostaglandin F receptor gene confers risk for essential hypertension in the han Chinese population. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2015 ; Vol. 35, No. 7. pp. 1687-1695.
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title = "Rare SNP rs12731181 in the miR-590-3p target site of the prostaglandin F2α receptor gene confers risk for essential hypertension in the han Chinese population",
abstract = "Objective-To investigate whether rs12731181 (A→G) interrupted miR-590-3p-mediated suppression of the prostaglandin F2α receptor (FP) and whether it is associated with essential hypertension in the Chinese population. Approach and Results-We found that miR-590-3p regulates human FP gene expression by binding to its 3′-untranslated region. rs12731181 (A→G) altered the binding affinity between miR-590-3p and its FP 3′-untranslated region target, thus reducing the suppression of FP expression, which, in turn, enhanced FP receptor-mediated contractility of vascular smooth muscle cells. Overexpression of FP augmented vascular tone and elevated blood pressure in mice. An association study was performed to analyze the relationship between the FP gene and essential hypertension in the Han Chinese population. The results indicated that the rs12731181 G allele was associated with susceptibility to essential hypertension. Carriers of the AG genotype exhibited significantly higher blood pressure than those of the AA genotype. FP gene expression was significantly higher in human peripheral leukocytes from individuals with the AG genotype than that in leukocytes from individuals with the AA genotype. Conclusions-rs12731181 in the seed region of the miR-590-3p target site is associated with increased risk of essential hypertension and represents a new paradigm for FP involvement in blood pressure regulation.",
keywords = "essential, hypertension, microRNAs, polymorphism, prostaglandin F<inf>2α</inf> receptor, single nucleotide",
author = "Bing Xiao and Gu, {Shui Ming} and Li, {Mulin Jun} and Jun Li and Bo Tao and Yuanyang Wang and Yan Wang and Shengkai Zuo and Yujun Shen and Yu Yu and Di Chen and Guilin Chen and Deping Kong and Juan Tang and Qian Liu and Chen, {Dong Rui} and Yong Liu and Sara Alberti and Melania Dovizio and Raffaele Landolfi and Luciana Mucci and Miao, {Pei Zhi} and Pingjin Gao and Zhu, {Ding Liang} and Junwen Wang and Bin Li and Paola Patrignani and Ying Yu",
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TY - JOUR

T1 - Rare SNP rs12731181 in the miR-590-3p target site of the prostaglandin F2α receptor gene confers risk for essential hypertension in the han Chinese population

AU - Xiao, Bing

AU - Gu, Shui Ming

AU - Li, Mulin Jun

AU - Li, Jun

AU - Tao, Bo

AU - Wang, Yuanyang

AU - Wang, Yan

AU - Zuo, Shengkai

AU - Shen, Yujun

AU - Yu, Yu

AU - Chen, Di

AU - Chen, Guilin

AU - Kong, Deping

AU - Tang, Juan

AU - Liu, Qian

AU - Chen, Dong Rui

AU - Liu, Yong

AU - Alberti, Sara

AU - Dovizio, Melania

AU - Landolfi, Raffaele

AU - Mucci, Luciana

AU - Miao, Pei Zhi

AU - Gao, Pingjin

AU - Zhu, Ding Liang

AU - Wang, Junwen

AU - Li, Bin

AU - Patrignani, Paola

AU - Yu, Ying

PY - 2015/7/27

Y1 - 2015/7/27

N2 - Objective-To investigate whether rs12731181 (A→G) interrupted miR-590-3p-mediated suppression of the prostaglandin F2α receptor (FP) and whether it is associated with essential hypertension in the Chinese population. Approach and Results-We found that miR-590-3p regulates human FP gene expression by binding to its 3′-untranslated region. rs12731181 (A→G) altered the binding affinity between miR-590-3p and its FP 3′-untranslated region target, thus reducing the suppression of FP expression, which, in turn, enhanced FP receptor-mediated contractility of vascular smooth muscle cells. Overexpression of FP augmented vascular tone and elevated blood pressure in mice. An association study was performed to analyze the relationship between the FP gene and essential hypertension in the Han Chinese population. The results indicated that the rs12731181 G allele was associated with susceptibility to essential hypertension. Carriers of the AG genotype exhibited significantly higher blood pressure than those of the AA genotype. FP gene expression was significantly higher in human peripheral leukocytes from individuals with the AG genotype than that in leukocytes from individuals with the AA genotype. Conclusions-rs12731181 in the seed region of the miR-590-3p target site is associated with increased risk of essential hypertension and represents a new paradigm for FP involvement in blood pressure regulation.

AB - Objective-To investigate whether rs12731181 (A→G) interrupted miR-590-3p-mediated suppression of the prostaglandin F2α receptor (FP) and whether it is associated with essential hypertension in the Chinese population. Approach and Results-We found that miR-590-3p regulates human FP gene expression by binding to its 3′-untranslated region. rs12731181 (A→G) altered the binding affinity between miR-590-3p and its FP 3′-untranslated region target, thus reducing the suppression of FP expression, which, in turn, enhanced FP receptor-mediated contractility of vascular smooth muscle cells. Overexpression of FP augmented vascular tone and elevated blood pressure in mice. An association study was performed to analyze the relationship between the FP gene and essential hypertension in the Han Chinese population. The results indicated that the rs12731181 G allele was associated with susceptibility to essential hypertension. Carriers of the AG genotype exhibited significantly higher blood pressure than those of the AA genotype. FP gene expression was significantly higher in human peripheral leukocytes from individuals with the AG genotype than that in leukocytes from individuals with the AA genotype. Conclusions-rs12731181 in the seed region of the miR-590-3p target site is associated with increased risk of essential hypertension and represents a new paradigm for FP involvement in blood pressure regulation.

KW - essential

KW - hypertension

KW - microRNAs

KW - polymorphism

KW - prostaglandin F<inf>2α</inf> receptor

KW - single nucleotide

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U2 - 10.1161/ATVBAHA.115.305445

DO - 10.1161/ATVBAHA.115.305445

M3 - Article

VL - 35

SP - 1687

EP - 1695

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 7

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