TY - JOUR
T1 - Rapid purification of intact tonofilaments from newborn rats
T2 - Comparison with glial filaments and neurofilaments
AU - Yen, Shu Hui
AU - Liem, Ronald K.H.
AU - Jenq, Lin Tai
AU - Shelanski, Michael L.
N1 - Funding Information:
This work was supported by grants from the National Institute of Neurological, Communicative Disorders and Stroke (NS-I 15041 and the McKnight Foundation. S.-H. Y. and R. K. H. L. were. respectively. Post-doctoral Trainee and Post-doctoral Fellow of the National Institute of Neurological. Communicative Disorders and Stroke.
PY - 1980
Y1 - 1980
N2 - A rapid, new procedure for the isolation of intact tonofilaments from newborn rat skins is described. The filament preparations show two major protein subunits on SDS-PAGE with molecular weights of 58000 and 66000 D. An antiserum prepared against the 58000 D protein reacted specifically with the tonofilament preparation, but not with the protein subunits of neurofilaments, glial filaments, tubulin or actin. This specificity is confirmed by indirect immunofluorescence: anti-P58 reacts with the epidermis, whereas antisera against the neurofilament or glial filament proteins and anti-tubulin do not. These data suggest that epidermal filaments represent a class of intermediate filaments distinct from either glial filaments or neurofilaments.
AB - A rapid, new procedure for the isolation of intact tonofilaments from newborn rat skins is described. The filament preparations show two major protein subunits on SDS-PAGE with molecular weights of 58000 and 66000 D. An antiserum prepared against the 58000 D protein reacted specifically with the tonofilament preparation, but not with the protein subunits of neurofilaments, glial filaments, tubulin or actin. This specificity is confirmed by indirect immunofluorescence: anti-P58 reacts with the epidermis, whereas antisera against the neurofilament or glial filament proteins and anti-tubulin do not. These data suggest that epidermal filaments represent a class of intermediate filaments distinct from either glial filaments or neurofilaments.
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U2 - 10.1016/0014-4827(80)90498-X
DO - 10.1016/0014-4827(80)90498-X
M3 - Article
C2 - 7000525
AN - SCOPUS:0019192277
SN - 0014-4827
VL - 129
SP - 313
EP - 320
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -