Rapid purification of intact tonofilaments from newborn rats. Comparison with glial filaments and neurofilaments

S. Yen, R. K H Liem, L. Jenq, M. L. Shelanski

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A rapid, new procedure for the isolation of intact tonofilaments from newborn rat skins is described. The filament preparations show two major protein subunits on SDS-PAGE with molecular weights of 58 000 and 66 000 D. An antiserum prepared against the 58 000 D protein reacted specifically with the tonofilament preparation, but not with the protein subunits of neurofilaments, glial filaments, tubulin or actin. This specificity is confirmed by indirect immunofluorescence: anti-P58 reacts with the epidermis, whereas antisera against the neurofilament or glial filament proteins and anti-tubulin do not. These data suggest that epidermal filaments represent a class of intermediate filaments distinct from either glial filaments or neurofilaments.

Original languageEnglish (US)
Pages (from-to)313-320
Number of pages8
JournalExperimental Cell Research
Volume129
Issue number2
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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