TY - JOUR
T1 - Rapid isolation of total acidic proteins from chromatin of various chick tissues
AU - Wilson, Elizabeth M.
AU - Spelsberg, Thomas C.
N1 - Funding Information:
The excellent technical assistance of Mrs Gayle Cashion is appreciated. This research is supported by Public Health Service Grants CA 13o65-Ol and CA 1492O-Ol from the National Cancer Institute. T.C.S. is a member of the Center for Population Research (USPHSHD 05797) at Vanderbi]t University School of Medicine.
PY - 1973/9/21
Y1 - 1973/9/21
N2 - A method is described for the rapid isolation of the nuclear acidic proteins of chromatin. Bovine pancreatic deoxyribonuclease I is used to hydrolyze DNA of dehistonized chromatin into acid-soluble fragments. The acidic proteins are recovered with more than 95% yield. Characterization of the isolated acidic proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis reveals reproducible heterogeneous banding patterns which are specific for the following chick tissues: liver, spleen, erythrocyte and heart, as well as oviduct at various stages of development. [3H]Ovalbumin added to dehistonized chromatin is not degraded throughout the procedure demonstrating that the multiple bands are not due to proteolytic activity.
AB - A method is described for the rapid isolation of the nuclear acidic proteins of chromatin. Bovine pancreatic deoxyribonuclease I is used to hydrolyze DNA of dehistonized chromatin into acid-soluble fragments. The acidic proteins are recovered with more than 95% yield. Characterization of the isolated acidic proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis reveals reproducible heterogeneous banding patterns which are specific for the following chick tissues: liver, spleen, erythrocyte and heart, as well as oviduct at various stages of development. [3H]Ovalbumin added to dehistonized chromatin is not degraded throughout the procedure demonstrating that the multiple bands are not due to proteolytic activity.
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U2 - 10.1016/0005-2795(73)90185-2
DO - 10.1016/0005-2795(73)90185-2
M3 - Article
C2 - 4744328
AN - SCOPUS:0015822225
SN - 0005-2795
VL - 322
SP - 145
EP - 154
JO - BBA - Protein Structure
JF - BBA - Protein Structure
IS - 1
ER -