Rapid isolation of total acidic proteins from chromatin of various chick tissues

Elizabeth M. Wilson, Thomas C. Spelsberg

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

A method is described for the rapid isolation of the nuclear acidic proteins of chromatin. Bovine pancreatic deoxyribonuclease I is used to hydrolyze DNA of dehistonized chromatin into acid-soluble fragments. The acidic proteins are recovered with more than 95% yield. Characterization of the isolated acidic proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis reveals reproducible heterogeneous banding patterns which are specific for the following chick tissues: liver, spleen, erythrocyte and heart, as well as oviduct at various stages of development. [3H]Ovalbumin added to dehistonized chromatin is not degraded throughout the procedure demonstrating that the multiple bands are not due to proteolytic activity.

Original languageEnglish (US)
Pages (from-to)145-154
Number of pages10
JournalBBA - Protein Structure
Volume322
Issue number1
DOIs
StatePublished - Sep 21 1973

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Rapid isolation of total acidic proteins from chromatin of various chick tissues'. Together they form a unique fingerprint.

  • Cite this