A method is described for the rapid isolation of the nuclear acidic proteins of chromatin. Bovine pancreatic deoxyribonuclease I is used to hydrolyze DNA of dehistonized chromatin into acid-soluble fragments. The acidic proteins are recovered with more than 95% yield. Characterization of the isolated acidic proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis reveals reproducible heterogeneous banding patterns which are specific for the following chick tissues: liver, spleen, erythrocyte and heart, as well as oviduct at various stages of development. [3H]Ovalbumin added to dehistonized chromatin is not degraded throughout the procedure demonstrating that the multiple bands are not due to proteolytic activity.
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