Rapid inhibition of the c-jun proto-oncogene expression in avian oviduct by estrogen

C. K. Lau, M. Subramaniam, K. Rasmussen, T. C. Spelsberg

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The c-jun proto-oncogene codes for an important component of the AP-1 transcription factor complex which regulates the expression of many genes. This apper demonstrates that the steady state c-jun mRNA level in the avian oviduct is markedly decreased to 50% of control values within 30 minutes after estrogen injection into the animals. In the avian liver, the level is rapidly increased over 10 fold. Nuclear run-off transcription analysis demonstrates that the decrease in the c-jun mRNA levels in the avian oviduct occurs at least in part at the level of transcription. These changes are reproducible in repeat experiments. This response in the avian oviduct is unique since 1) it is the first demonstration of a steroid effect on c-jun expression in any animal system, 2) the changes in c-jun mRNA occur much more rapidly than most steroid responsive genes, and 3) it is the rare demonstration of an estrogen inhibition of the expression of a gene. A role for the c-jun proto-oncogene as an early regulatory gene in the cascade model for steroid action is proposed.

Original languageEnglish (US)
Pages (from-to)2595-2597
Number of pages3
JournalEndocrinology
Volume127
Issue number5
StatePublished - 1990

Fingerprint

jun Genes
Oviducts
Estrogens
Steroids
Messenger RNA
Gene Expression
Transcription Factor AP-1
Regulator Genes
Injections
Liver
Genes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Lau, C. K., Subramaniam, M., Rasmussen, K., & Spelsberg, T. C. (1990). Rapid inhibition of the c-jun proto-oncogene expression in avian oviduct by estrogen. Endocrinology, 127(5), 2595-2597.

Rapid inhibition of the c-jun proto-oncogene expression in avian oviduct by estrogen. / Lau, C. K.; Subramaniam, M.; Rasmussen, K.; Spelsberg, T. C.

In: Endocrinology, Vol. 127, No. 5, 1990, p. 2595-2597.

Research output: Contribution to journalArticle

Lau, CK, Subramaniam, M, Rasmussen, K & Spelsberg, TC 1990, 'Rapid inhibition of the c-jun proto-oncogene expression in avian oviduct by estrogen', Endocrinology, vol. 127, no. 5, pp. 2595-2597.
Lau CK, Subramaniam M, Rasmussen K, Spelsberg TC. Rapid inhibition of the c-jun proto-oncogene expression in avian oviduct by estrogen. Endocrinology. 1990;127(5):2595-2597.
Lau, C. K. ; Subramaniam, M. ; Rasmussen, K. ; Spelsberg, T. C. / Rapid inhibition of the c-jun proto-oncogene expression in avian oviduct by estrogen. In: Endocrinology. 1990 ; Vol. 127, No. 5. pp. 2595-2597.
@article{a78e9bc06f804dfdb370420b7eabe7eb,
title = "Rapid inhibition of the c-jun proto-oncogene expression in avian oviduct by estrogen",
abstract = "The c-jun proto-oncogene codes for an important component of the AP-1 transcription factor complex which regulates the expression of many genes. This apper demonstrates that the steady state c-jun mRNA level in the avian oviduct is markedly decreased to 50{\%} of control values within 30 minutes after estrogen injection into the animals. In the avian liver, the level is rapidly increased over 10 fold. Nuclear run-off transcription analysis demonstrates that the decrease in the c-jun mRNA levels in the avian oviduct occurs at least in part at the level of transcription. These changes are reproducible in repeat experiments. This response in the avian oviduct is unique since 1) it is the first demonstration of a steroid effect on c-jun expression in any animal system, 2) the changes in c-jun mRNA occur much more rapidly than most steroid responsive genes, and 3) it is the rare demonstration of an estrogen inhibition of the expression of a gene. A role for the c-jun proto-oncogene as an early regulatory gene in the cascade model for steroid action is proposed.",
author = "Lau, {C. K.} and M. Subramaniam and K. Rasmussen and Spelsberg, {T. C.}",
year = "1990",
language = "English (US)",
volume = "127",
pages = "2595--2597",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "5",

}

TY - JOUR

T1 - Rapid inhibition of the c-jun proto-oncogene expression in avian oviduct by estrogen

AU - Lau, C. K.

AU - Subramaniam, M.

AU - Rasmussen, K.

AU - Spelsberg, T. C.

PY - 1990

Y1 - 1990

N2 - The c-jun proto-oncogene codes for an important component of the AP-1 transcription factor complex which regulates the expression of many genes. This apper demonstrates that the steady state c-jun mRNA level in the avian oviduct is markedly decreased to 50% of control values within 30 minutes after estrogen injection into the animals. In the avian liver, the level is rapidly increased over 10 fold. Nuclear run-off transcription analysis demonstrates that the decrease in the c-jun mRNA levels in the avian oviduct occurs at least in part at the level of transcription. These changes are reproducible in repeat experiments. This response in the avian oviduct is unique since 1) it is the first demonstration of a steroid effect on c-jun expression in any animal system, 2) the changes in c-jun mRNA occur much more rapidly than most steroid responsive genes, and 3) it is the rare demonstration of an estrogen inhibition of the expression of a gene. A role for the c-jun proto-oncogene as an early regulatory gene in the cascade model for steroid action is proposed.

AB - The c-jun proto-oncogene codes for an important component of the AP-1 transcription factor complex which regulates the expression of many genes. This apper demonstrates that the steady state c-jun mRNA level in the avian oviduct is markedly decreased to 50% of control values within 30 minutes after estrogen injection into the animals. In the avian liver, the level is rapidly increased over 10 fold. Nuclear run-off transcription analysis demonstrates that the decrease in the c-jun mRNA levels in the avian oviduct occurs at least in part at the level of transcription. These changes are reproducible in repeat experiments. This response in the avian oviduct is unique since 1) it is the first demonstration of a steroid effect on c-jun expression in any animal system, 2) the changes in c-jun mRNA occur much more rapidly than most steroid responsive genes, and 3) it is the rare demonstration of an estrogen inhibition of the expression of a gene. A role for the c-jun proto-oncogene as an early regulatory gene in the cascade model for steroid action is proposed.

UR - http://www.scopus.com/inward/record.url?scp=0025059423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025059423&partnerID=8YFLogxK

M3 - Article

C2 - 2121466

AN - SCOPUS:0025059423

VL - 127

SP - 2595

EP - 2597

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 5

ER -