Rapid genetic analysis of families with polycystic kidney disease 1 by means of a microsatellite marker

P. C. Harris, S. Thomas, P. J. Ratcliffe, M. H. Breuning, E. Coto, C. Lopez-Larrea

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Presymptomatic diagnosis of polycystic kidney disease 1 (PKD1) is possible by genetic linkage analysis with markers from both sides of the disease locus. The existing proximal markers are not informative in many families, so such analysis is difficult and time-consuming. We sought more useful length polymorphisms on the proximal side of the locus among simple sequence repeats (microsatellites). We identified two microsatellite polymorphisms that lie closer to the PKD1 locus than any previously described highly variable marker. One, SM7, is especially informative; we have found fourteen alleles and the observed heterozygosity in caucasians is 62·7%. Genetic linkage analysis in PKD1 families suggests that both of the markers lie proximal to the disease gene, closer than existing flanking markers. These polymorphisms can be simply assayed by polymerase chain reaction amplification of the variable regions, which generates DNA fragments that can be separated on non-denaturing acrylamide gels and directly examined after gel staining. This rapid, inexpensive, and non-radioactive method of linkage analysis allows the complete study of DNA samples within 8 h.

Original languageEnglish (US)
Pages (from-to)1484-1487
Number of pages4
JournalThe Lancet
Volume338
Issue number8781
DOIs
StatePublished - Dec 14 1991

    Fingerprint

ASJC Scopus subject areas

  • Medicine(all)

Cite this