Rapid endocytosis coupled to exocytosis in adrenal chromaffin cells involves Ca2+, GTP, and dynamin but not clathrin

Cristina R. Artalejo, John R Henley, Mark A Mc Niven, H. Clive Palfrey

Research output: Contribution to journalArticle

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Abstract

Rapid endocytosis (RE) occurs immediately after an exocytotic burst in adrenal chromaffin cells. Capacitance measurements of endocytosis reveal that recovery of membrane is a biphasic process that is complete within 20 sec. The ultimate extent of membrane retrieval is precisely controlled and capacitance invariably returns to its prestimulation value. The mechanism of RE specifically requires intracellular Ca2+; Sr2+ and Ba2+ do not substitute, although all three cations support secretion. Thus the divalent cation receptors for RE and exocytosis must be distinct molecules. RE is dependent on GTP hydrolysis; it is blocked by GTP removal or replacement with guanosine 5′-[γ-thio]triphosphate. In the presence of GTP, multiple rounds of secretion followed by RE could be elicited from the same cell. RE requires participation of dynamin, a guanine nucleotide binding protein, as revealed by intracellular immunological antagonism of this protein. Intact microtubules may be essential, as nocodazole also blocked RE. Whereas anti-dynamin antibodies blocked RE, anti-clathrin antibodies did not, suggesting that clathrin-coated vesicles are not involved in this form of endocytosis. RE may represent the initial step in the rapid recycling of secretory granules in the chromaffin cell.

Original languageEnglish (US)
Pages (from-to)8328-8332
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number18
StatePublished - Aug 29 1995

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Dynamins
Clathrin
Chromaffin Cells
Exocytosis
Endocytosis
Guanosine Triphosphate
Anti-Idiotypic Antibodies
Clathrin-Coated Vesicles
Nocodazole
Guanine Nucleotides
Membranes
Guanosine
Divalent Cations
Secretory Vesicles
Microtubules
Cations
Carrier Proteins
Hydrolysis

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Rapid endocytosis coupled to exocytosis in adrenal chromaffin cells involves Ca2+, GTP, and dynamin but not clathrin. / Artalejo, Cristina R.; Henley, John R; Mc Niven, Mark A; Palfrey, H. Clive.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 18, 29.08.1995, p. 8328-8332.

Research output: Contribution to journalArticle

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AU - Palfrey, H. Clive

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