Rapid downregulation of NCX and PMCA in hippocampal neurons following H2O2 oxidative stress

Sertac N. Kip, Emanuel E. Strehler

Research output: Chapter in Book/Report/Conference proceedingConference contribution

20 Citations (Scopus)

Abstract

Na+/Ca2+ exchangers (NCXs) and plasma membrane Ca2+ pumps (PMCAs) are crucial for intracellular Ca2+ homeostasis and Ca2+ signaling. Elevated [Ca2+] i is a hallmark of neurodegenerative disease and stroke. Here we studied the short-term effect of oxidative stress on the plasma membrane Ca 2+ extrusion systems in hippocampal neurons (HN) and found that after 2-3 h exposure to 300 μM H2O2, all NCXs and PMCAs were significantly downregulated at the RNA (NCX) and protein (PMCA) level. Rapid internalization and aggregation of the PMCA was also observed. Our data show that the plasma membrane calcium extrusion systems are sensitive early targets of neurotoxic oxidative stress.

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages436-439
Number of pages4
Volume1099
DOIs
StatePublished - Mar 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1099
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Oxidative stress
Cell membranes
Neurons
Oxidative Stress
Down-Regulation
Cell Membrane
Pumps
Extrusion
Neurodegenerative diseases
Neurodegenerative Diseases
Homeostasis
Agglomeration
Stroke
RNA
Calcium
Proteins

Keywords

  • Calcium extrusion
  • Hippocampal neurons
  • Na/Ca exchanger
  • NCX
  • Oxidative stress
  • PMCA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kip, S. N., & Strehler, E. E. (2007). Rapid downregulation of NCX and PMCA in hippocampal neurons following H2O2 oxidative stress. In Annals of the New York Academy of Sciences (Vol. 1099, pp. 436-439). (Annals of the New York Academy of Sciences; Vol. 1099). https://doi.org/10.1196/annals.1387.005

Rapid downregulation of NCX and PMCA in hippocampal neurons following H2O2 oxidative stress. / Kip, Sertac N.; Strehler, Emanuel E.

Annals of the New York Academy of Sciences. Vol. 1099 2007. p. 436-439 (Annals of the New York Academy of Sciences; Vol. 1099).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kip, SN & Strehler, EE 2007, Rapid downregulation of NCX and PMCA in hippocampal neurons following H2O2 oxidative stress. in Annals of the New York Academy of Sciences. vol. 1099, Annals of the New York Academy of Sciences, vol. 1099, pp. 436-439. https://doi.org/10.1196/annals.1387.005
Kip SN, Strehler EE. Rapid downregulation of NCX and PMCA in hippocampal neurons following H2O2 oxidative stress. In Annals of the New York Academy of Sciences. Vol. 1099. 2007. p. 436-439. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1387.005
Kip, Sertac N. ; Strehler, Emanuel E. / Rapid downregulation of NCX and PMCA in hippocampal neurons following H2O2 oxidative stress. Annals of the New York Academy of Sciences. Vol. 1099 2007. pp. 436-439 (Annals of the New York Academy of Sciences).
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