Rapid determination of serum 5-fluorocytosine levels by high-performance liquid chromatography

R. B. Diasio, M. E. Wilburn, S. Shadomy, A. Espinel-Ingroff

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

A method for the rapid quantitative determination of 5-fluorocytosine (5-FC) in serum by high-performance liquid chromatography (HPLC) has been developed. After initially ultrafiltrating the serum, a portion was injected onto a cation exchange column. 5 FC was separated by using an ammonium-phosphate buffer as the mobile phase and detected by ultraviolet absorption at 254 nm. Quantitation of 5-FC was based on the linear relationship between peak area in the chromatograms and known concentrations of 5-FC in a set of serum standards (prepared by adding from 10 to 200 μg of 5-FC to 1-ml aliquots of pooled human serum). This method was compared with the standard microbiological method for 5-FC. Advantages of the HPLC method include: determination of 5-FC levels within 30 min; lack of interference from other antimicrobial drugs, particularly amphotericin B; more accurate determination of true 5-FC level, particularly at concentrations of <25 μg/ml or >100 μg/ml; and ease with which the assay may be automated for routine use.

Original languageEnglish (US)
Pages (from-to)5OO-504
JournalAntimicrobial Agents and Chemotherapy
Volume13
Issue number3
DOIs
StatePublished - 1978

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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