Rapid characterization of candidate biomarkers for pancreatic cancer using cell microarrays (CMAs)

Min Sik Kim, Sarada V. Kuppireddy, Sruthi Sakamuri, Mukul Singal, Derese Getnet, H. C. Harsha, Renu Goel, Lavanya Balakrishnan, Harrys K.C. Jacob, Manoj K. Kashyap, Shantal G. Tankala, Anirban Maitra, Christine A. Iacobuzio-Donahue, Elizabeth Jaffee, Michael G. Goggins, Victor E. Velculescu, Ralph H. Hruban, Akhilesh Pandey

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Tissue microarrays have become a valuable tool for high-throughput analysis using immunohistochemical labeling. However, the large majority of biochemical studies are carried out in cell lines to further characterize candidate biomarkers or therapeutic targets with subsequent studies in animals or using primary tissues. Thus, cell line-based microarrays could be a useful screening tool in some situations. Here, we constructed a cell microarray (CMA) containing a panel of 40 pancreatic cancer cell lines available from American Type Culture Collection in addition to those locally available at Johns Hopkins. As proof of principle, we performed immunocytochemical labeling of an epithelial cell adhesion molecule (Ep-CAM), a molecule generally expressed in the epithelium, on this pancreatic cancer CMA. In addition, selected molecules that have been previously shown to be differentially expressed in pancreatic cancer in the literature were validated. For example, we observed strong labeling of CA19-9 antigen, a prognostic and predictive marker for pancreatic cancer. We also carried out a bioinformatics analysis of a literature curated catalog of pancreatic cancer biomarkers developed previously by our group and identified two candidate biomarkers, HLA class I and transmembrane protease, serine 4 (TMPRSS4), and examined their expression in the cell lines represented on the pancreatic cancer CMAs. Our results demonstrate the utility of CMAs as a useful resource for rapid screening of molecules of interest and suggest that CMAs can become a universal standard platform in cancer research.

Original languageEnglish (US)
Pages (from-to)5556-5563
Number of pages8
JournalJournal of Proteome Research
Volume11
Issue number11
DOIs
StatePublished - Nov 2 2012
Externally publishedYes

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Biomarkers
Microarrays
Pancreatic Neoplasms
Cells
Cell Line
Labeling
Molecules
Screening
Tissue
Serine Proteases
Tumor Biomarkers
Computational Biology
Cell Adhesion Molecules
Bioinformatics
Cell culture
Epithelium
Animals
Antigens
Throughput
Research

Keywords

  • biomarkers
  • immunocytochemistry
  • immunohistochemistry
  • microarrays

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Rapid characterization of candidate biomarkers for pancreatic cancer using cell microarrays (CMAs). / Kim, Min Sik; Kuppireddy, Sarada V.; Sakamuri, Sruthi; Singal, Mukul; Getnet, Derese; Harsha, H. C.; Goel, Renu; Balakrishnan, Lavanya; Jacob, Harrys K.C.; Kashyap, Manoj K.; Tankala, Shantal G.; Maitra, Anirban; Iacobuzio-Donahue, Christine A.; Jaffee, Elizabeth; Goggins, Michael G.; Velculescu, Victor E.; Hruban, Ralph H.; Pandey, Akhilesh.

In: Journal of Proteome Research, Vol. 11, No. 11, 02.11.2012, p. 5556-5563.

Research output: Contribution to journalArticle

Kim, MS, Kuppireddy, SV, Sakamuri, S, Singal, M, Getnet, D, Harsha, HC, Goel, R, Balakrishnan, L, Jacob, HKC, Kashyap, MK, Tankala, SG, Maitra, A, Iacobuzio-Donahue, CA, Jaffee, E, Goggins, MG, Velculescu, VE, Hruban, RH & Pandey, A 2012, 'Rapid characterization of candidate biomarkers for pancreatic cancer using cell microarrays (CMAs)', Journal of Proteome Research, vol. 11, no. 11, pp. 5556-5563. https://doi.org/10.1021/pr300483r
Kim, Min Sik ; Kuppireddy, Sarada V. ; Sakamuri, Sruthi ; Singal, Mukul ; Getnet, Derese ; Harsha, H. C. ; Goel, Renu ; Balakrishnan, Lavanya ; Jacob, Harrys K.C. ; Kashyap, Manoj K. ; Tankala, Shantal G. ; Maitra, Anirban ; Iacobuzio-Donahue, Christine A. ; Jaffee, Elizabeth ; Goggins, Michael G. ; Velculescu, Victor E. ; Hruban, Ralph H. ; Pandey, Akhilesh. / Rapid characterization of candidate biomarkers for pancreatic cancer using cell microarrays (CMAs). In: Journal of Proteome Research. 2012 ; Vol. 11, No. 11. pp. 5556-5563.
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AU - Kim, Min Sik

AU - Kuppireddy, Sarada V.

AU - Sakamuri, Sruthi

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AU - Getnet, Derese

AU - Harsha, H. C.

AU - Goel, Renu

AU - Balakrishnan, Lavanya

AU - Jacob, Harrys K.C.

AU - Kashyap, Manoj K.

AU - Tankala, Shantal G.

AU - Maitra, Anirban

AU - Iacobuzio-Donahue, Christine A.

AU - Jaffee, Elizabeth

AU - Goggins, Michael G.

AU - Velculescu, Victor E.

AU - Hruban, Ralph H.

AU - Pandey, Akhilesh

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