Rapid assay of HSF1 and HSF2 gene expression by RT-PCR

Xuan Z. Ding, Robert C. Smallridge, Richard J. Galloway, Juliann G. Kiang

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.

Original languageEnglish (US)
Pages (from-to)189-192
Number of pages4
JournalMolecular and Cellular Biochemistry
Volume158
Issue number2
DOIs
StatePublished - Jan 1 1996

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Keywords

  • Epithelia
  • Gene
  • HSF1
  • HSF2
  • Heat shock proteins
  • Method
  • Reverse transcriptase polymerase chain reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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