Rapid assay of HSF1 and HSF2 gene expression by RT-PCR

Xuan Z. Ding; Robert C. Smallridge; Richard J. Galloway; Juliann G. Kiang

doi:10.1007/BF00225845

Rapid assay of HSF1 and HSF2 gene expression by RT-PCR

Xuan Z. Ding, Robert C. Smallridge, Richard J. Galloway, Juliann G. Kiang

Endocrinology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.

Original language	English (US)
Pages (from-to)	189-192
Number of pages	4
Journal	Molecular and Cellular Biochemistry
Volume	158
Issue number	2
DOIs	https://doi.org/10.1007/BF00225845
State	Published - 1996

Keywords

Epithelia
Gene
HSF1
HSF2
Heat shock proteins
Method
Reverse transcriptase polymerase chain reaction

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1007/BF00225845

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title = "Rapid assay of HSF1 and HSF2 gene expression by RT-PCR",

abstract = "Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.",

keywords = "Epithelia, Gene, HSF1, HSF2, Heat shock proteins, Method, Reverse transcriptase polymerase chain reaction",

author = "Ding, {Xuan Z.} and Smallridge, {Robert C.} and Galloway, {Richard J.} and Kiang, {Juliann G.}",

year = "1996",

doi = "10.1007/BF00225845",

language = "English (US)",

volume = "158",

pages = "189--192",

journal = "Molecular and Cellular Biochemistry",

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T1 - Rapid assay of HSF1 and HSF2 gene expression by RT-PCR

AU - Ding, Xuan Z.

AU - Smallridge, Robert C.

AU - Galloway, Richard J.

AU - Kiang, Juliann G.

PY - 1996

Y1 - 1996

N2 - Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.

AB - Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.

KW - Epithelia

KW - Gene

KW - HSF1

KW - HSF2

KW - Heat shock proteins

KW - Method

KW - Reverse transcriptase polymerase chain reaction

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JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

IS - 2

ER -

Rapid assay of HSF1 and HSF2 gene expression by RT-PCR

Abstract

Keywords

ASJC Scopus subject areas

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