Rapid assay of HSF1 and HSF2 gene expression by RT-PCR

Xuan Z. Ding, Robert C. Smallridge, Richard J. Galloway, Juliann G. Kiang

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.

Original languageEnglish (US)
Pages (from-to)189-192
Number of pages4
JournalMolecular and Cellular Biochemistry
Volume158
Issue number2
DOIs
StatePublished - Jan 1 1996

Keywords

  • Epithelia
  • Gene
  • HSF1
  • HSF2
  • Heat shock proteins
  • Method
  • Reverse transcriptase polymerase chain reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Rapid assay of HSF1 and HSF2 gene expression by RT-PCR'. Together they form a unique fingerprint.

  • Cite this

    Ding, X. Z., Smallridge, R. C., Galloway, R. J., & Kiang, J. G. (1996). Rapid assay of HSF1 and HSF2 gene expression by RT-PCR. Molecular and Cellular Biochemistry, 158(2), 189-192. https://doi.org/10.1007/BF00225845