Rapid and simultaneous detection of genes encoding klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in gram-negative bacilli

Scott A. Cunningham, Tabassum Noorie, Daniele Meunier, Neil Woodford, Robin Patel

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Wepresent a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-=- lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.

Original languageEnglish (US)
Pages (from-to)1269-1271
Number of pages3
JournalJournal of clinical microbiology
Volume51
Issue number4
DOIs
StatePublished - Apr 1 2013

ASJC Scopus subject areas

  • Microbiology (medical)

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