Ragweed-induced expression of GATA-3, IL-4, and IL-5 by eosinophils in the lungs of allergic C57BL/6J mice

J. Paul Justice, M. T. Borchers, J. J. Lee, W. H. Rowan, Y. Shibata, M. R. Van Scott

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Allergen-induced recruitment of T lymphocytes and eosinophils to the airways is associated with increased expression of the transcription factor GATA-3. In this study, the relationship between airway inflammation and GATA-3 expression in the lungs was investigated using ragweed-sensitized C57BL/6J mice. Intratracheal ragweed challenge increased both the number of GATA-3-expressing cells in the perivascular and peribronchial regions and the amount of expression per cell. Interleukin (IL)-4 and IL-5 levels in bronchoalveolar lavage fluid were upregulated in parallel with GATA-3 expression. GATA-3 mRNA and protein colocalized to eosinophils. Eosinophils isolated from the lungs and stimulated with phorbol 12-myristate 13-acetate and/or A-23187 released IL-5. The release was inhibited by actinomycin D, which indicates that de novo synthesis of the cytokine was involved. Western blot analysis of proteins from isolated eosinophils demonstrated expression of the p50 subunit of nuclear factor-κB, a transcription factor that is implicated in control of GATA-3 expression. These data provide evidence that allergen challenge increases GATA-3 and proinflammatory cytokine expression by pulmonary eosinophils, which could provide positive feedback for the inflammatory response.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume282
Issue number2 26-2
StatePublished - 2002

Fingerprint

Ambrosia
Interleukin-5
Inbred C57BL Mouse
Eosinophils
Interleukin-4
Lung
Allergens
Transcription Factor 3
Cytokines
Calcimycin
Bronchoalveolar Lavage Fluid
Dactinomycin
Proteins
Acetates
Transcription Factors
Western Blotting
Inflammation
T-Lymphocytes
Messenger RNA

Keywords

  • Airway inflammation
  • Asthma
  • Interleukin
  • Major basic protein
  • Transcription

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Paul Justice, J., Borchers, M. T., Lee, J. J., Rowan, W. H., Shibata, Y., & Van Scott, M. R. (2002). Ragweed-induced expression of GATA-3, IL-4, and IL-5 by eosinophils in the lungs of allergic C57BL/6J mice. American Journal of Physiology - Lung Cellular and Molecular Physiology, 282(2 26-2).

Ragweed-induced expression of GATA-3, IL-4, and IL-5 by eosinophils in the lungs of allergic C57BL/6J mice. / Paul Justice, J.; Borchers, M. T.; Lee, J. J.; Rowan, W. H.; Shibata, Y.; Van Scott, M. R.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 282, No. 2 26-2, 2002.

Research output: Contribution to journalArticle

Paul Justice, J. ; Borchers, M. T. ; Lee, J. J. ; Rowan, W. H. ; Shibata, Y. ; Van Scott, M. R. / Ragweed-induced expression of GATA-3, IL-4, and IL-5 by eosinophils in the lungs of allergic C57BL/6J mice. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2002 ; Vol. 282, No. 2 26-2.
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