RAG-1 and Ly6D Independently Reflect Progression in the B Lymphoid Lineage

Qingzhao Zhang, Brandt L. Esplin, Ryuji Iida, Karla P. Garrett, Zhixin L. Huang, Kay L Medina, Paul W. Kincade

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Common lymphoid progenitors (CLPs) are thought to represent major intermediates in the transition of hematopoietic stem cells (HSCs) to B lineage lymphocytes. However, it has been obvious for some time that CLPs are heterogeneous, and there has been controversy concerning their differentiation potential. We have now resolved four Flt3+ CLP subsets that are relatively homogenous and capable of forming B cells. Differentiation potential and gene expression patterns suggest Flt3+ CLPs lacking both Ly6D and RAG-1 are the least differentiated. In addition to B cells, they generate natural killer (NK) and dendritic cells (DCs). At the other extreme is a subset of the recently described Flt3+ Ly6D+ CLPs that have a history of RAG-1 expression and are B lineage restricted. These relatively abundant and potent CLPs were depleted within 48 hours of acute in vivo estrogen elevation, suggesting they descend from hormone regulated progenitors. This contrasts with the hormone insensitivity of other CLP subsets that include NK lineage progenitors. This progenitor heterogeneity and differentiation complexity may add flexibility in response to environmental changes. Expression of RAG-1 and display of Ly6D are both milestone events, but they are neither synchronized nor dependent on each other.

Original languageEnglish (US)
Article numbere72397
JournalPLoS One
Volume8
Issue number8
DOIs
StatePublished - Aug 30 2013

Fingerprint

Lymphoid Progenitor Cells
B-lymphocytes
Cells
hormones
Hormones
Lymphocytes
natural killer cells
dendritic cells
Stem cells
Gene expression
estrogens
Estrogens
lymphocytes
Display devices
gene expression
B-Lymphocytes
Hematopoietic Stem Cells
Natural Killer Cells
Dendritic Cells
Gene Expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Zhang, Q., Esplin, B. L., Iida, R., Garrett, K. P., Huang, Z. L., Medina, K. L., & Kincade, P. W. (2013). RAG-1 and Ly6D Independently Reflect Progression in the B Lymphoid Lineage. PLoS One, 8(8), [e72397]. https://doi.org/10.1371/journal.pone.0072397

RAG-1 and Ly6D Independently Reflect Progression in the B Lymphoid Lineage. / Zhang, Qingzhao; Esplin, Brandt L.; Iida, Ryuji; Garrett, Karla P.; Huang, Zhixin L.; Medina, Kay L; Kincade, Paul W.

In: PLoS One, Vol. 8, No. 8, e72397, 30.08.2013.

Research output: Contribution to journalArticle

Zhang, Q, Esplin, BL, Iida, R, Garrett, KP, Huang, ZL, Medina, KL & Kincade, PW 2013, 'RAG-1 and Ly6D Independently Reflect Progression in the B Lymphoid Lineage', PLoS One, vol. 8, no. 8, e72397. https://doi.org/10.1371/journal.pone.0072397
Zhang, Qingzhao ; Esplin, Brandt L. ; Iida, Ryuji ; Garrett, Karla P. ; Huang, Zhixin L. ; Medina, Kay L ; Kincade, Paul W. / RAG-1 and Ly6D Independently Reflect Progression in the B Lymphoid Lineage. In: PLoS One. 2013 ; Vol. 8, No. 8.
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