Radiometric assay of ghrelin hydrolase activity and 3H-ghrelin distribution into mouse tissues

Vicky Ping Chen, Yang Gao, Liyi Geng, Stephen Brimijoin

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A high-throughput radiometric assay was developed to characterize enzymatic hydrolysis of ghrelin and to track the peptide's fate in vivo. The assay is based on solvent partitioning of [3H]-octanoic acid liberated from [3H]-octanoyl ghrelin during enzymatic hydrolysis. This simple and cost-effective method facilitates kinetic analysis of ghrelin hydrolase activity of native and mutated butyrylcholinesterases or carboxylesterases from multiple species. In addition, the assay's high sensitivity facilitates ready evaluation of ghrelin's pharmacokinetics and tissue distribution in mice after i.v. bolus administration of radiolabeled peptide.

Original languageEnglish (US)
Pages (from-to)732-739
Number of pages8
JournalBiochemical Pharmacology
Volume98
Issue number4
DOIs
StatePublished - Dec 15 2015

Keywords

  • Butyrylcholinesterase
  • Enzyme kinetics
  • Ghrelin
  • Pharmacokinetics
  • Radiometric assay

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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