Radioimmunoassay for rat B-type natriuretic peptide (BNP-45)

I. R. Sarda, D. A. Wigle, Dennis A Wigle, S. C. Pang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Rat BNP-45 is the main circulating form of BNP in rat plasma. To understand the role of BNP in physiological and pathophysiological conditions, a specific radioimmunoassay (RIA) for the quantitative determination of the peptide in plasma and tissues is necessary. An assay using rBNP-45 as the standard in conjunction with antisera directed against this peptide has not been described in the literature, though some investigators have reported values ranging from 0.73-2.0 pmol/L using either BNP-26 or BNP-32 as the standard peptide. Unfortunately, these forms of BNP do not exist in rat plasma. In our studies, we have developed a specific RIA for rBNP-45 using rBNP-45 as the standard peptide and Tyr(o)-rBNP-45 as the radioligand. We have used two specific antisera for assay purposes; one against rBNP-45, and the second to a peptide composed of the first 20 amino acids of rBNP-45 (rBNP[1-20]). The recovery of various amounts of rBNP-45 added to control plasma was 50-80% depending on the method of extraction and purification. The interassay and intraassay coefficients of variation were 12% and 6% respectively. Values obtained were similar for blood sampled by either cardiac puncture, decapitation, or aortic puncture. The method was used to measure rBNP-45 in the plasma of normal (WKY) and Spontaneously Hypertensive (SHR) rats. The values obtained were 5.46 ± 0.43 and 19.6 ± 2.36 pmol/L respectively. The rat atrial natriuretic peptide (ANP[99-126]) values in the same extracts were 23.2 ± 0.45 and 51.6 ± 3.16 pmol/L.

Original languageEnglish (US)
Pages (from-to)167-182
Number of pages16
JournalJournal of Immunoassay
Volume14
Issue number3
StatePublished - 1993
Externally publishedYes

Fingerprint

Brain Natriuretic Peptide
Radioimmunoassay
Rats
Plasmas
Peptides
Punctures
Immune Sera
Assays
Decapitation
Atrial Natriuretic Factor
Inbred SHR Rats
Purification
Blood
Research Personnel
brain natriuretic peptide-45
Tissue
Amino Acids
Recovery

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Sarda, I. R., Wigle, D. A., Wigle, D. A., & Pang, S. C. (1993). Radioimmunoassay for rat B-type natriuretic peptide (BNP-45). Journal of Immunoassay, 14(3), 167-182.

Radioimmunoassay for rat B-type natriuretic peptide (BNP-45). / Sarda, I. R.; Wigle, D. A.; Wigle, Dennis A; Pang, S. C.

In: Journal of Immunoassay, Vol. 14, No. 3, 1993, p. 167-182.

Research output: Contribution to journalArticle

Sarda, IR, Wigle, DA, Wigle, DA & Pang, SC 1993, 'Radioimmunoassay for rat B-type natriuretic peptide (BNP-45)', Journal of Immunoassay, vol. 14, no. 3, pp. 167-182.
Sarda, I. R. ; Wigle, D. A. ; Wigle, Dennis A ; Pang, S. C. / Radioimmunoassay for rat B-type natriuretic peptide (BNP-45). In: Journal of Immunoassay. 1993 ; Vol. 14, No. 3. pp. 167-182.
@article{eec349a82f874588aeefac021b3fbbb5,
title = "Radioimmunoassay for rat B-type natriuretic peptide (BNP-45)",
abstract = "Rat BNP-45 is the main circulating form of BNP in rat plasma. To understand the role of BNP in physiological and pathophysiological conditions, a specific radioimmunoassay (RIA) for the quantitative determination of the peptide in plasma and tissues is necessary. An assay using rBNP-45 as the standard in conjunction with antisera directed against this peptide has not been described in the literature, though some investigators have reported values ranging from 0.73-2.0 pmol/L using either BNP-26 or BNP-32 as the standard peptide. Unfortunately, these forms of BNP do not exist in rat plasma. In our studies, we have developed a specific RIA for rBNP-45 using rBNP-45 as the standard peptide and Tyr(o)-rBNP-45 as the radioligand. We have used two specific antisera for assay purposes; one against rBNP-45, and the second to a peptide composed of the first 20 amino acids of rBNP-45 (rBNP[1-20]). The recovery of various amounts of rBNP-45 added to control plasma was 50-80{\%} depending on the method of extraction and purification. The interassay and intraassay coefficients of variation were 12{\%} and 6{\%} respectively. Values obtained were similar for blood sampled by either cardiac puncture, decapitation, or aortic puncture. The method was used to measure rBNP-45 in the plasma of normal (WKY) and Spontaneously Hypertensive (SHR) rats. The values obtained were 5.46 ± 0.43 and 19.6 ± 2.36 pmol/L respectively. The rat atrial natriuretic peptide (ANP[99-126]) values in the same extracts were 23.2 ± 0.45 and 51.6 ± 3.16 pmol/L.",
author = "Sarda, {I. R.} and Wigle, {D. A.} and Wigle, {Dennis A} and Pang, {S. C.}",
year = "1993",
language = "English (US)",
volume = "14",
pages = "167--182",
journal = "Journal of Immunoassay and Immunochemistry",
issn = "1532-1819",
publisher = "Taylor and Francis Ltd.",
number = "3",

}

TY - JOUR

T1 - Radioimmunoassay for rat B-type natriuretic peptide (BNP-45)

AU - Sarda, I. R.

AU - Wigle, D. A.

AU - Wigle, Dennis A

AU - Pang, S. C.

PY - 1993

Y1 - 1993

N2 - Rat BNP-45 is the main circulating form of BNP in rat plasma. To understand the role of BNP in physiological and pathophysiological conditions, a specific radioimmunoassay (RIA) for the quantitative determination of the peptide in plasma and tissues is necessary. An assay using rBNP-45 as the standard in conjunction with antisera directed against this peptide has not been described in the literature, though some investigators have reported values ranging from 0.73-2.0 pmol/L using either BNP-26 or BNP-32 as the standard peptide. Unfortunately, these forms of BNP do not exist in rat plasma. In our studies, we have developed a specific RIA for rBNP-45 using rBNP-45 as the standard peptide and Tyr(o)-rBNP-45 as the radioligand. We have used two specific antisera for assay purposes; one against rBNP-45, and the second to a peptide composed of the first 20 amino acids of rBNP-45 (rBNP[1-20]). The recovery of various amounts of rBNP-45 added to control plasma was 50-80% depending on the method of extraction and purification. The interassay and intraassay coefficients of variation were 12% and 6% respectively. Values obtained were similar for blood sampled by either cardiac puncture, decapitation, or aortic puncture. The method was used to measure rBNP-45 in the plasma of normal (WKY) and Spontaneously Hypertensive (SHR) rats. The values obtained were 5.46 ± 0.43 and 19.6 ± 2.36 pmol/L respectively. The rat atrial natriuretic peptide (ANP[99-126]) values in the same extracts were 23.2 ± 0.45 and 51.6 ± 3.16 pmol/L.

AB - Rat BNP-45 is the main circulating form of BNP in rat plasma. To understand the role of BNP in physiological and pathophysiological conditions, a specific radioimmunoassay (RIA) for the quantitative determination of the peptide in plasma and tissues is necessary. An assay using rBNP-45 as the standard in conjunction with antisera directed against this peptide has not been described in the literature, though some investigators have reported values ranging from 0.73-2.0 pmol/L using either BNP-26 or BNP-32 as the standard peptide. Unfortunately, these forms of BNP do not exist in rat plasma. In our studies, we have developed a specific RIA for rBNP-45 using rBNP-45 as the standard peptide and Tyr(o)-rBNP-45 as the radioligand. We have used two specific antisera for assay purposes; one against rBNP-45, and the second to a peptide composed of the first 20 amino acids of rBNP-45 (rBNP[1-20]). The recovery of various amounts of rBNP-45 added to control plasma was 50-80% depending on the method of extraction and purification. The interassay and intraassay coefficients of variation were 12% and 6% respectively. Values obtained were similar for blood sampled by either cardiac puncture, decapitation, or aortic puncture. The method was used to measure rBNP-45 in the plasma of normal (WKY) and Spontaneously Hypertensive (SHR) rats. The values obtained were 5.46 ± 0.43 and 19.6 ± 2.36 pmol/L respectively. The rat atrial natriuretic peptide (ANP[99-126]) values in the same extracts were 23.2 ± 0.45 and 51.6 ± 3.16 pmol/L.

UR - http://www.scopus.com/inward/record.url?scp=0027157227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027157227&partnerID=8YFLogxK

M3 - Article

C2 - 8354718

AN - SCOPUS:0027157227

VL - 14

SP - 167

EP - 182

JO - Journal of Immunoassay and Immunochemistry

JF - Journal of Immunoassay and Immunochemistry

SN - 1532-1819

IS - 3

ER -