Radiation-induced lung injury in vivo: Expression of transforming growth factor-beta precedes fibrosis

Eunhee S. Yi; Adriana Bedoya; Hyesun Lee; Elaine Chin; William Saunders; Seong Jin Kim; David Danielpour; Daniel G. Remick; Songmei Yin; Thomas R. Ulich

doi:10.1007/BF01486737

Radiation-induced lung injury in vivo: Expression of transforming growth factor-beta precedes fibrosis

Eunhee S. Yi, Adriana Bedoya, Hyesun Lee, Elaine Chin, William Saunders, Seong Jin Kim, David Danielpour, Daniel G. Remick, Songmei Yin, Thomas R. Ulich

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

Cytokine release from irradiated cells has been postulated to start soon after irradiation preceding detectable clinical and pathological manifestation of lung injury. The expression of transforming growth factor beta (TGFβ), a fibrogenic and radiation-inducible cytokine, was studied from 1-16 weeks after the 15 and 30 Gray (Gy) of thoracic irradiation to rats. Thoracic irradiation caused an increase in TGFβ protein in bronchoalveolar lavage (BAL) fluid peaking at 3-6 weeks as compared to sham-irradiated control rats. Steady state TGFβ mRNA expression as shown by whole lung northern blot assay paralleled the TGFβ protein expression in BAL fluid. The peak of TGFβ protein increase in BAL fluid between 3 and 6 weeks coincided with the initial influx of inflammatory cells in BAL fluid, but preceded histologically discernable pulmonary fibrosis that was not apparent until 8-10 weeks after irradiation. In conclusion, TGFβ and mRNA and protein upregulation preceded the radiation-induced pulmonary fibrosis, suggesting a pathogenetic role in the development of radiation fibrosis.

Original language	English (US)
Pages (from-to)	339-352
Number of pages	14
Journal	Inflammation
Volume	20
Issue number	4
DOIs	https://doi.org/10.1007/BF01486737
State	Published - 1996

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Radiation-induced lung injury in vivo: Expression of transforming growth factor-beta precedes fibrosis",

abstract = "Cytokine release from irradiated cells has been postulated to start soon after irradiation preceding detectable clinical and pathological manifestation of lung injury. The expression of transforming growth factor beta (TGFβ), a fibrogenic and radiation-inducible cytokine, was studied from 1-16 weeks after the 15 and 30 Gray (Gy) of thoracic irradiation to rats. Thoracic irradiation caused an increase in TGFβ protein in bronchoalveolar lavage (BAL) fluid peaking at 3-6 weeks as compared to sham-irradiated control rats. Steady state TGFβ mRNA expression as shown by whole lung northern blot assay paralleled the TGFβ protein expression in BAL fluid. The peak of TGFβ protein increase in BAL fluid between 3 and 6 weeks coincided with the initial influx of inflammatory cells in BAL fluid, but preceded histologically discernable pulmonary fibrosis that was not apparent until 8-10 weeks after irradiation. In conclusion, TGFβ and mRNA and protein upregulation preceded the radiation-induced pulmonary fibrosis, suggesting a pathogenetic role in the development of radiation fibrosis.",

author = "Yi, {Eunhee S.} and Adriana Bedoya and Hyesun Lee and Elaine Chin and William Saunders and Kim, {Seong Jin} and David Danielpour and Remick, {Daniel G.} and Songmei Yin and Ulich, {Thomas R.}",

year = "1996",

doi = "10.1007/BF01486737",

language = "English (US)",

volume = "20",

pages = "339--352",

journal = "Inflammation",

issn = "0360-3997",

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T1 - Radiation-induced lung injury in vivo

T2 - Expression of transforming growth factor-beta precedes fibrosis

AU - Yi, Eunhee S.

AU - Bedoya, Adriana

AU - Lee, Hyesun

AU - Chin, Elaine

AU - Saunders, William

AU - Kim, Seong Jin

AU - Danielpour, David

AU - Remick, Daniel G.

AU - Yin, Songmei

AU - Ulich, Thomas R.

PY - 1996

Y1 - 1996

N2 - Cytokine release from irradiated cells has been postulated to start soon after irradiation preceding detectable clinical and pathological manifestation of lung injury. The expression of transforming growth factor beta (TGFβ), a fibrogenic and radiation-inducible cytokine, was studied from 1-16 weeks after the 15 and 30 Gray (Gy) of thoracic irradiation to rats. Thoracic irradiation caused an increase in TGFβ protein in bronchoalveolar lavage (BAL) fluid peaking at 3-6 weeks as compared to sham-irradiated control rats. Steady state TGFβ mRNA expression as shown by whole lung northern blot assay paralleled the TGFβ protein expression in BAL fluid. The peak of TGFβ protein increase in BAL fluid between 3 and 6 weeks coincided with the initial influx of inflammatory cells in BAL fluid, but preceded histologically discernable pulmonary fibrosis that was not apparent until 8-10 weeks after irradiation. In conclusion, TGFβ and mRNA and protein upregulation preceded the radiation-induced pulmonary fibrosis, suggesting a pathogenetic role in the development of radiation fibrosis.

AB - Cytokine release from irradiated cells has been postulated to start soon after irradiation preceding detectable clinical and pathological manifestation of lung injury. The expression of transforming growth factor beta (TGFβ), a fibrogenic and radiation-inducible cytokine, was studied from 1-16 weeks after the 15 and 30 Gray (Gy) of thoracic irradiation to rats. Thoracic irradiation caused an increase in TGFβ protein in bronchoalveolar lavage (BAL) fluid peaking at 3-6 weeks as compared to sham-irradiated control rats. Steady state TGFβ mRNA expression as shown by whole lung northern blot assay paralleled the TGFβ protein expression in BAL fluid. The peak of TGFβ protein increase in BAL fluid between 3 and 6 weeks coincided with the initial influx of inflammatory cells in BAL fluid, but preceded histologically discernable pulmonary fibrosis that was not apparent until 8-10 weeks after irradiation. In conclusion, TGFβ and mRNA and protein upregulation preceded the radiation-induced pulmonary fibrosis, suggesting a pathogenetic role in the development of radiation fibrosis.

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JO - Inflammation

JF - Inflammation

IS - 4

ER -

Radiation-induced lung injury in vivo: Expression of transforming growth factor-beta precedes fibrosis

Abstract

ASJC Scopus subject areas

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