Rabgap1 promotes recycling of active β1 integrins to support effective cell migration

Anna V. Samarelli, Tilman Ziegler, Alexander Meves, Reinhard Fässler, Ralph T. Böttcher

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Integrin function depends on the continuous internalization of integrins and their subsequent endosomal recycling to the plasma membrane to drive adhesion dynamics, cell migration and invasion. Here we assign a pivotal role for Rabgap1 (GAPCenA) in the recycling of endocytosed active β1 integrins to the plasma membrane. The phosphotyrosine-binding (PTB) domain of Rabgap1 binds to the membrane-proximal NPxY motif in the cytoplasmic domain of β1 integrin subunits on endosomes. Silencing Rabgap1 in mouse fibroblasts leads to the intracellular accumulation of active β1 integrins, alters focal adhesion formation, and decreases cell migration and cancer cell invasion. Functionally, Rabgap1 facilitates active β1 integrin recycling to the plasma membrane through attenuation of Rab11 activity. Taken together, our results identify Rabgap1 as an important factor for conformation-specific integrin trafficking and define the role of Rabgap1 in β1-integrin-mediated cell migration in mouse fibroblasts and breast cancer cells.

Original languageEnglish (US)
Article numberjcs243683
JournalJournal of cell science
Volume133
Issue number18
DOIs
StatePublished - Sep 2020

Keywords

  • Active β1 integrins
  • Cancer cell invasion
  • Cell migration
  • Integrin recycling
  • Rabgap1

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Rabgap1 promotes recycling of active β1 integrins to support effective cell migration'. Together they form a unique fingerprint.

Cite this