Quantitative signature for architectural organization of regulatory factors using intranuclear informatics

Daniel W. Young, Sayyed K. Zaidi, Paul S. Furcinitti, Amjad Javed, Andre J. van Wijnen, Janet L. Stein, Jane B. Lian, Gary S. Stein

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Regulatory machinery for replication and gene expression is punctately organized in supramolecular complexes that are compartmentalized in nuclear microenvironments. Quantitative approaches are required to understand the assembly of regulatory machinery within the context of nuclear architecture and to provide a mechanistic link with biological control. We have developed 'intranuclear informatics' to quantify functionally relevant parameters of spatially organized nuclear domains. Using this informatics strategy we have characterized post-mitotic re-establishment of focal subnuclear organization of Runx (AML/Cbfa) transcription factors in progeny cells. By analyzing point mutations that abrogate fidelity of Runx intranuclear targeting, we establish molecular determinants for the spatial order of Runx domains. Our novel approach provides evidence that architectural organization of Runx factors may be fundamental to their tissue-specific regulatory function.

Original languageEnglish (US)
Pages (from-to)4889-4896
Number of pages8
JournalJournal of cell science
Volume117
Issue number21
DOIs
StatePublished - Oct 1 2004

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Keywords

  • Informatics
  • Nuclear architecture
  • Quantitative
  • Runx (AML/Cbfa)
  • Transcription

ASJC Scopus subject areas

  • Cell Biology

Cite this

Young, D. W., Zaidi, S. K., Furcinitti, P. S., Javed, A., van Wijnen, A. J., Stein, J. L., Lian, J. B., & Stein, G. S. (2004). Quantitative signature for architectural organization of regulatory factors using intranuclear informatics. Journal of cell science, 117(21), 4889-4896. https://doi.org/10.1242/jcs.01229