Quantitative real-time polymerase chain reaction for evaluating DNAemia due to cytomegalovirus, Epstein-Barr virus, and BK virus in solid-organ transplant recipients

Thomas F. Smith, Mark J. Espy, Jayawant Mandrekar, Mary F. Jones, Franklin R. Cockerill, Robin Patel

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Testing for cytomegalovirus-, Epstein-Barr virus-, and BK virus-specific gene targets in specimens from solid-organ transplant recipients for DNA by quantitative real-time polymerase chain reaction has been implemented in many diagnostic facilities. This technology provides rapid, accurate, and reproducible results for early detection, monitoring, and medical management of patients with these infections. Because these assays are becoming commonly used in clinical practice, the technical variables associated with specimen processing (e.g., nucleic acid extraction, gene target, and result reporting), amplification, and unique patient characteristics (e.g., age, sex, underlying diseases, immune status, and immunosuppressive regimens received) are factors that may influence the understanding and interpretation of test results. We emphasize the need for standardization of existing variables through parallel comparative and proficiency testing, uniform units for expressing results, to provide for clinical correlation with the results of these molecular assays.

Original languageEnglish (US)
Pages (from-to)1056-1061
Number of pages6
JournalClinical Infectious Diseases
Volume45
Issue number8
DOIs
StatePublished - 2007

Fingerprint

BK Virus
Cytomegalovirus
Human Herpesvirus 4
Real-Time Polymerase Chain Reaction
Transplants
Immune System Diseases
Immunosuppressive Agents
Nucleic Acids
Genes
Technology
DNA
Infection
Transplant Recipients

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Quantitative real-time polymerase chain reaction for evaluating DNAemia due to cytomegalovirus, Epstein-Barr virus, and BK virus in solid-organ transplant recipients. / Smith, Thomas F.; Espy, Mark J.; Mandrekar, Jayawant; Jones, Mary F.; Cockerill, Franklin R.; Patel, Robin.

In: Clinical Infectious Diseases, Vol. 45, No. 8, 2007, p. 1056-1061.

Research output: Contribution to journalArticle

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