@article{a929bebaeaa9450bb774ddc7e3d3edd0,
title = "Quantitative proteomics of urinary bladder cancer cell lines identify uap1 as a potential therapeutic target",
abstract = "Bladder carcinoma (BC) incidence and mortality rates are increasing worldwide. The development of novel therapeutic strategies is required to improve clinical management of this cancer. Aberrant protein expression may lead to cancer initiation and progression. Therefore, the identification of these potential protein targets and limiting their expression levels would provide alternative treatment options. In this study, we utilized a liquid-chromatography tandem mass spectrometry-based global proteomics approach to identify differentially expressed proteins in bladder cancer cell lines. A total of 3913 proteins were identified in this study, of which 479 proteins were overexpressed and 141 proteins were downregulated in 4 out of 6 BC cell lines when compared with normal human urothelial cell line (TERT-NHUC). We evaluated the role of UDP-N-acetylhexosamine pyrophosphorylase (UAP1) in bladder cancer pathogenesis. The silencing of UAP1 led to reduction in proliferation, invasion, colony formation and migration capability of bladder cancer cell lines. Thus, our study reveals UAP1 as a promising therapeutic target for bladder cancer.",
keywords = "Molecular subtypes, Quantitative proteomics, Therapeutic target, Urothelial carcinoma",
author = "Puttamallesh, {Vinuth N.} and Barnali Deb and Kirti Gondkar and Ankit Jain and Bipin Nair and Akhilesh Pandey and Aditi Chatterjee and Harsha Gowda and Prashant Kumar",
note = "Funding Information: This research was funded by Department of Science and Technology (DST), Ramanujan Fellowship, Government of India, grant number SB/S2/RJN-077/2015, and the APC was funded by SB/S2/RJN-077/2015, Department of Science and Technology (DST), Ramanujan Fellowship, Government of India. Funding Information: Acknowledgments: We thank Jean Paul Thiery (Department of Biochemistry, National University of Singapore, Singapore) for providing bladder cancer cell lines and normal human urothelial cell line TERT-NHUC (JTERT). We thank the Department of Biotechnology (DBT), Government of India, for research support to the Institute of Bioinformatics (IOB), Bangalore. We thank the “Manipal Academy of Higher Education”, Madhav Nagar, Manipal 576104, India, for research support to the Institute of Bioinformatics. Harsha Gowda is a Wellcome trust/DBT India Alliance Intermediate carrier Fellow. Prashant Kumar is a recipient of the Ramanujan Fellowship awarded by the Department of Science and Technology (DST), Government of India. BD is a recipient of the INSPIRE Fellowship, Department of Science and Technology, Government of India. KG is a recipient of the Senior Research Fellowship from the University Grants Commission (UGC), Government of India. Funding Information: Funding: This research was funded by Department of Science and Technology (DST), Ramanujan Fellowship, Government of India, grant number SB/S2/RJN-077/2015, and the APC was funded by SB/S2/RJN-077/2015, Department of Science and Technology (DST), Ramanujan Fellowship, Government of India. Publisher Copyright: {\textcopyright} 2020 by the authors. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = jul,
doi = "10.3390/genes11070763",
language = "English (US)",
volume = "11",
pages = "1--17",
journal = "Genes",
issn = "2073-4425",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "7",
}