Quantitative proteomics of urinary bladder cancer cell lines identify uap1 as a potential therapeutic target

Vinuth N. Puttamallesh, Barnali Deb, Kirti Gondkar, Ankit Jain, Bipin Nair, Akhilesh Pandey, Aditi Chatterjee, Harsha Gowda, Prashant Kumar

Research output: Contribution to journalArticlepeer-review

Abstract

Bladder carcinoma (BC) incidence and mortality rates are increasing worldwide. The development of novel therapeutic strategies is required to improve clinical management of this cancer. Aberrant protein expression may lead to cancer initiation and progression. Therefore, the identification of these potential protein targets and limiting their expression levels would provide alternative treatment options. In this study, we utilized a liquid-chromatography tandem mass spectrometry-based global proteomics approach to identify differentially expressed proteins in bladder cancer cell lines. A total of 3913 proteins were identified in this study, of which 479 proteins were overexpressed and 141 proteins were downregulated in 4 out of 6 BC cell lines when compared with normal human urothelial cell line (TERT-NHUC). We evaluated the role of UDP-N-acetylhexosamine pyrophosphorylase (UAP1) in bladder cancer pathogenesis. The silencing of UAP1 led to reduction in proliferation, invasion, colony formation and migration capability of bladder cancer cell lines. Thus, our study reveals UAP1 as a promising therapeutic target for bladder cancer.

Original languageEnglish (US)
Article number763
Pages (from-to)1-17
Number of pages17
JournalGenes
Volume11
Issue number7
DOIs
StatePublished - Jul 2020

Keywords

  • Molecular subtypes
  • Quantitative proteomics
  • Therapeutic target
  • Urothelial carcinoma

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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