Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway

Jun Zhong, Marissa Martinez, Srona Sengupta, Albert Lee, Xinyan Wu, Raghothama Chaerkady, Aditi Chatterjee, Robert N. O'Meally, Robert N. Cole, Akhilesh Pandey, Natasha E. Zachara

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The modification of intracellular proteins by monosaccharides of O-linked β-N-acetylglucosamine (O-GlcNAc) is an essential and dynamic PTM of metazoans. The addition and removal of O-GlcNAc is catalyzed by the O-GlcNAc transferase (OGT) and O-GlcNAcase, respectively. One mechanism by which O-GlcNAc is thought to mediate proteins is by regulating phosphorylation. To provide insight into the pathways regulated by O-GlcNAc, we have utilized SILAC-based quantitative proteomics to carry out comparisons of site-specific phosphorylation in OGT wild-type and Null cells. Quantitation of the phosphoproteome demonstrated that of 5529 phosphoserine, phosphothreonine, and phosphotyrosine sites, 232 phosphosites were upregulated and 133 downregulated in the absence of O-GlcNAc. Collectively, these data suggest that deletion of OGT has a profound effect on the phosphorylation of cell cycle and DNA damage response proteins. Key events were confirmed by biochemical analyses and demonstrate an increase in the activating autophosphorylation event on ATM (Ser1987) and on ATM's downstream targets p53, H2AX, and Chk2. Together, these data support widespread changes in the phosphoproteome upon removal of O-GlcNAc, suggesting that O-GlcNAc regulates processes such as the cell cycle, genomic stability, and lysosomal biogenesis. All MS data have been deposited in the ProteomeXchange with identifier PXD001153 (http://proteomecentral.proteomexchange.org/dataset/PXD001153).

Original languageEnglish (US)
Pages (from-to)591-607
Number of pages17
JournalProteomics
Volume15
Issue number2-3
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Phosphorylation
Crosstalk
DNA Damage
Automatic teller machines
DNA
Cell Cycle
Cells
Phosphothreonine
Pulse time modulation
Phosphoserine
Null Lymphocytes
Phosphotyrosine
Proteins
Acetylglucosamine
Monosaccharides
Genomic Instability
Proteomics
Down-Regulation
O-GlcNAc transferase

Keywords

  • ATM
  • Cell biology
  • Glycobiology
  • Glycosylation
  • OGT
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway. / Zhong, Jun; Martinez, Marissa; Sengupta, Srona; Lee, Albert; Wu, Xinyan; Chaerkady, Raghothama; Chatterjee, Aditi; O'Meally, Robert N.; Cole, Robert N.; Pandey, Akhilesh; Zachara, Natasha E.

In: Proteomics, Vol. 15, No. 2-3, 01.01.2015, p. 591-607.

Research output: Contribution to journalArticle

Zhong, J, Martinez, M, Sengupta, S, Lee, A, Wu, X, Chaerkady, R, Chatterjee, A, O'Meally, RN, Cole, RN, Pandey, A & Zachara, NE 2015, 'Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway', Proteomics, vol. 15, no. 2-3, pp. 591-607. https://doi.org/10.1002/pmic.201400339
Zhong, Jun ; Martinez, Marissa ; Sengupta, Srona ; Lee, Albert ; Wu, Xinyan ; Chaerkady, Raghothama ; Chatterjee, Aditi ; O'Meally, Robert N. ; Cole, Robert N. ; Pandey, Akhilesh ; Zachara, Natasha E. / Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway. In: Proteomics. 2015 ; Vol. 15, No. 2-3. pp. 591-607.
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abstract = "The modification of intracellular proteins by monosaccharides of O-linked β-N-acetylglucosamine (O-GlcNAc) is an essential and dynamic PTM of metazoans. The addition and removal of O-GlcNAc is catalyzed by the O-GlcNAc transferase (OGT) and O-GlcNAcase, respectively. One mechanism by which O-GlcNAc is thought to mediate proteins is by regulating phosphorylation. To provide insight into the pathways regulated by O-GlcNAc, we have utilized SILAC-based quantitative proteomics to carry out comparisons of site-specific phosphorylation in OGT wild-type and Null cells. Quantitation of the phosphoproteome demonstrated that of 5529 phosphoserine, phosphothreonine, and phosphotyrosine sites, 232 phosphosites were upregulated and 133 downregulated in the absence of O-GlcNAc. Collectively, these data suggest that deletion of OGT has a profound effect on the phosphorylation of cell cycle and DNA damage response proteins. Key events were confirmed by biochemical analyses and demonstrate an increase in the activating autophosphorylation event on ATM (Ser1987) and on ATM's downstream targets p53, H2AX, and Chk2. Together, these data support widespread changes in the phosphoproteome upon removal of O-GlcNAc, suggesting that O-GlcNAc regulates processes such as the cell cycle, genomic stability, and lysosomal biogenesis. All MS data have been deposited in the ProteomeXchange with identifier PXD001153 (http://proteomecentral.proteomexchange.org/dataset/PXD001153).",
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AU - Zhong, Jun

AU - Martinez, Marissa

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AU - Lee, Albert

AU - Wu, Xinyan

AU - Chaerkady, Raghothama

AU - Chatterjee, Aditi

AU - O'Meally, Robert N.

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AU - Pandey, Akhilesh

AU - Zachara, Natasha E.

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AB - The modification of intracellular proteins by monosaccharides of O-linked β-N-acetylglucosamine (O-GlcNAc) is an essential and dynamic PTM of metazoans. The addition and removal of O-GlcNAc is catalyzed by the O-GlcNAc transferase (OGT) and O-GlcNAcase, respectively. One mechanism by which O-GlcNAc is thought to mediate proteins is by regulating phosphorylation. To provide insight into the pathways regulated by O-GlcNAc, we have utilized SILAC-based quantitative proteomics to carry out comparisons of site-specific phosphorylation in OGT wild-type and Null cells. Quantitation of the phosphoproteome demonstrated that of 5529 phosphoserine, phosphothreonine, and phosphotyrosine sites, 232 phosphosites were upregulated and 133 downregulated in the absence of O-GlcNAc. Collectively, these data suggest that deletion of OGT has a profound effect on the phosphorylation of cell cycle and DNA damage response proteins. Key events were confirmed by biochemical analyses and demonstrate an increase in the activating autophosphorylation event on ATM (Ser1987) and on ATM's downstream targets p53, H2AX, and Chk2. Together, these data support widespread changes in the phosphoproteome upon removal of O-GlcNAc, suggesting that O-GlcNAc regulates processes such as the cell cycle, genomic stability, and lysosomal biogenesis. All MS data have been deposited in the ProteomeXchange with identifier PXD001153 (http://proteomecentral.proteomexchange.org/dataset/PXD001153).

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