Quantitative phosphoproteomic analysis reveals reciprocal activation of receptor tyrosine kinases between cancer epithelial cells and stromal fibroblasts

Xinyan Wu, Muhammad Saddiq Zahari, Santosh Renuse, Nandini A. Sahasrabuddhe, Raghothama Chaerkady, Min Sik Kim, Mary Jo Fackler, Martha Stampfer, Edward Gabrielson, Saraswati Sukumar, Akhilesh Pandey

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Cancer-associated fibroblasts (CAFs) are one of the most important components of tumor stroma and play a key role in modulating tumor growth. However, a mechanistic understanding of how CAFs communicate with tumor cells to promote their proliferation and invasion is far from complete. A major reason for this is that most current techniques and model systems do not capture the complexity of signal transduction that occurs between CAFs and tumor cells. Methods: In this study, we employed a stable isotope labeling with amino acids in cell culture (SILAC) strategy to label invasive breast cancer cells, MDA-MB-231, and breast cancer patient-derived CAF this has already been defined above cells. We used an antibody-based phosphotyrosine peptide enrichment method coupled to LC-MS/MS to catalog and quantify tyrosine phosphorylation-mediated signal transduction events induced by the bidirectional communication between patient-derived CAFs and tumor cells. Results: We discovered that distinct signaling events were activated in CAFs and in tumor epithelial cells during the crosstalk between these two cell types. We identified reciprocal activation of a number of receptor tyrosine kinases including EGFR, FGFR1 and EPHA2 induced by this bidirectional communication. Conclusions: Our study not only provides insights into the mechanisms of the interaction between CAFs and tumor cells, but the model system described here could be used as a prototype for analysis of intercellular communication in many different tumor microenvironments.

Original languageEnglish (US)
Article number21
JournalClinical Proteomics
Volume15
Issue number1
DOIs
StatePublished - Jun 15 2018
Externally publishedYes

Fingerprint

Receptor Protein-Tyrosine Kinases
Fibroblasts
Tumors
Epithelial Cells
Chemical activation
Chemical analysis
Cells
Neoplasms
Signal transduction
Signal Transduction
Communication
Breast Neoplasms
Isotope Labeling
Phosphotyrosine
Tumor Microenvironment
Phosphorylation
Cancer-Associated Fibroblasts
Crosstalk
Tyrosine
Cell culture

Keywords

  • Breast cancer
  • Carcinoma-associated fibroblast
  • Co-culture
  • Epithelial cell
  • Mass spectrometry
  • Phosphoproteome
  • Signaling crosstalk
  • SILAC

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Quantitative phosphoproteomic analysis reveals reciprocal activation of receptor tyrosine kinases between cancer epithelial cells and stromal fibroblasts. / Wu, Xinyan; Zahari, Muhammad Saddiq; Renuse, Santosh; Sahasrabuddhe, Nandini A.; Chaerkady, Raghothama; Kim, Min Sik; Fackler, Mary Jo; Stampfer, Martha; Gabrielson, Edward; Sukumar, Saraswati; Pandey, Akhilesh.

In: Clinical Proteomics, Vol. 15, No. 1, 21, 15.06.2018.

Research output: Contribution to journalArticle

Wu, Xinyan ; Zahari, Muhammad Saddiq ; Renuse, Santosh ; Sahasrabuddhe, Nandini A. ; Chaerkady, Raghothama ; Kim, Min Sik ; Fackler, Mary Jo ; Stampfer, Martha ; Gabrielson, Edward ; Sukumar, Saraswati ; Pandey, Akhilesh. / Quantitative phosphoproteomic analysis reveals reciprocal activation of receptor tyrosine kinases between cancer epithelial cells and stromal fibroblasts. In: Clinical Proteomics. 2018 ; Vol. 15, No. 1.
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AU - Chaerkady, Raghothama

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AB - Background: Cancer-associated fibroblasts (CAFs) are one of the most important components of tumor stroma and play a key role in modulating tumor growth. However, a mechanistic understanding of how CAFs communicate with tumor cells to promote their proliferation and invasion is far from complete. A major reason for this is that most current techniques and model systems do not capture the complexity of signal transduction that occurs between CAFs and tumor cells. Methods: In this study, we employed a stable isotope labeling with amino acids in cell culture (SILAC) strategy to label invasive breast cancer cells, MDA-MB-231, and breast cancer patient-derived CAF this has already been defined above cells. We used an antibody-based phosphotyrosine peptide enrichment method coupled to LC-MS/MS to catalog and quantify tyrosine phosphorylation-mediated signal transduction events induced by the bidirectional communication between patient-derived CAFs and tumor cells. Results: We discovered that distinct signaling events were activated in CAFs and in tumor epithelial cells during the crosstalk between these two cell types. We identified reciprocal activation of a number of receptor tyrosine kinases including EGFR, FGFR1 and EPHA2 induced by this bidirectional communication. Conclusions: Our study not only provides insights into the mechanisms of the interaction between CAFs and tumor cells, but the model system described here could be used as a prototype for analysis of intercellular communication in many different tumor microenvironments.

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