TY - JOUR
T1 - Quantitative imaging of microvascular permeability in a rat model of lipopolysaccharide-induced sepsis
T2 - Evaluation using cryostatic microcomputed tomography
AU - Langheinrich, Alexander C.
AU - Ritman, Erik L.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/8
Y1 - 2006/8
N2 - OBJECTIVES: The aim of the present study was to evaluate the magnitude of endothelial defects by microcomputed tomography (CT) quantitation of contrast agent diffusion across the vascular endothelium in a rat model of vascular permeability caused by lipopolysaccharide (LPS)-induced sepsis. MATERIALS AND METHODS: LPS was administered intraperitoneally (i.p.) at a dose of 10 mg/kg body weight in male Wistar rats (n = 18). Vascular leakage and vascular volume were quantified, by micro-CT and cryostatic micro-CT. The contrast agents used were Fenestra (∼70-nm particle diameter), Microfil (large polymer), and iopamidol (Isovue, MW 777 Dalton). RESULTS: Micro-CT revealed an increase in endothelial permeability as indicated by entry of contrast agent (Fenestra) into the extravascular space after LPS administration (P < 0.01). Endotoxin exposure also induced a decrease of vascular luminal volume in the myocardium, liver, kidney, and colonic wall determined by micro-CT (P < 0.01). Vascular leakage, expressed as the ratio of extravascular to intravascular gray scale intensity (IE/II) after injection of contrast agent, increased significantly in the myocardium, liver, kidney, and colonic wall (P < 0.001). The elimination of iopamidol from the intravascular compartment in LPS-challenged rats was decreased compared with control rats. The endothelial defect size was estimated to be >70 nm and <1 μm. CONCLUSION: Contrast agents are useful to characterize vascular leakage and vascular volume fraction in an animal model of endotoxin priming.
AB - OBJECTIVES: The aim of the present study was to evaluate the magnitude of endothelial defects by microcomputed tomography (CT) quantitation of contrast agent diffusion across the vascular endothelium in a rat model of vascular permeability caused by lipopolysaccharide (LPS)-induced sepsis. MATERIALS AND METHODS: LPS was administered intraperitoneally (i.p.) at a dose of 10 mg/kg body weight in male Wistar rats (n = 18). Vascular leakage and vascular volume were quantified, by micro-CT and cryostatic micro-CT. The contrast agents used were Fenestra (∼70-nm particle diameter), Microfil (large polymer), and iopamidol (Isovue, MW 777 Dalton). RESULTS: Micro-CT revealed an increase in endothelial permeability as indicated by entry of contrast agent (Fenestra) into the extravascular space after LPS administration (P < 0.01). Endotoxin exposure also induced a decrease of vascular luminal volume in the myocardium, liver, kidney, and colonic wall determined by micro-CT (P < 0.01). Vascular leakage, expressed as the ratio of extravascular to intravascular gray scale intensity (IE/II) after injection of contrast agent, increased significantly in the myocardium, liver, kidney, and colonic wall (P < 0.001). The elimination of iopamidol from the intravascular compartment in LPS-challenged rats was decreased compared with control rats. The endothelial defect size was estimated to be >70 nm and <1 μm. CONCLUSION: Contrast agents are useful to characterize vascular leakage and vascular volume fraction in an animal model of endotoxin priming.
KW - Contrast agent
KW - Imaging
KW - Micro-CT
KW - Sepsis
KW - Vascular leakage
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U2 - 10.1097/01.rli.0000227494.17444.64
DO - 10.1097/01.rli.0000227494.17444.64
M3 - Article
C2 - 16829748
AN - SCOPUS:33747840899
SN - 0020-9996
VL - 41
SP - 645
EP - 650
JO - Investigative radiology
JF - Investigative radiology
IS - 8
ER -