Quantitative CT imaging of the spatio-temporal distribution patterns of vasa vasorum in aortas of apoE-/-/LDL-/- double knockout mice

M. Kampschulte, A. Brinkmann, P. Stieger, D. G. Sedding, C. Dierkes, R. M. Bohle, G. Krombach, Erik L. Ritman, A. C. Langheinrich

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Objective: To investigate the distribution of vasa vasorum (VV) relative to advanced atherosclerotic lesions (calcified, fibrotic or hemorrhaged) along the aortic wall of apoE-/-/LDL-/- mice at the age of 25 and 80 weeks using high-resolution nano-CT. Methods: Aortas from male apoE-/-/LDL-/- mice at the age of 25 weeks (n=4) and 80 weeks (n=7) were infused in situ with contrast agent and harvested for scanning with nano-CT. The spatial distribution of vasa vasorum [number and area/cross-section (mm2)] was compared to aortic luminal cross-sectional area and plaque cross-sectional area in the ascending aorta, aortic arch and descending aorta. Results were complemented with co-localized histology. Results: The number and total luminal cross-sectional area of VV showed a significant decrease in the ascending aorta and aortic arch from 25 to 80 weeks but not in the descending aorta. The number and cross-sectional area of VV showed significant local differences depending on whether it was near a fibrotic, and hemorrhaged or calcified plaque in animals at the age of 80 weeks. Area of VV progressively increased along the aorta from least in the ascending aorta < aortic arch < descending aorta in animals at the age of 80 weeks and is inverse in animals aged 25 weeks. Conclusion: Atherosclerotic lesion type is correlated to the number and cross-sectional area of VV in different aortic segments in apoE-/-/LDL-/- mice. The chronological development of VV along the aorta proceeds distally from the ascending aorta and aortic arch.

Original languageEnglish (US)
Pages (from-to)444-450
Number of pages7
JournalAtherosclerosis
Volume212
Issue number2
DOIs
StatePublished - Oct 1 2010

Keywords

  • Atherosclerosis
  • Imaging
  • Micro-CT
  • Nano-CT
  • Vasa vasorum

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Quantitative CT imaging of the spatio-temporal distribution patterns of vasa vasorum in aortas of apoE<sup>-/-</sup>/LDL<sup>-/-</sup> double knockout mice'. Together they form a unique fingerprint.

  • Cite this

    Kampschulte, M., Brinkmann, A., Stieger, P., Sedding, D. G., Dierkes, C., Bohle, R. M., Krombach, G., Ritman, E. L., & Langheinrich, A. C. (2010). Quantitative CT imaging of the spatio-temporal distribution patterns of vasa vasorum in aortas of apoE-/-/LDL-/- double knockout mice. Atherosclerosis, 212(2), 444-450. https://doi.org/10.1016/j.atherosclerosis.2010.07.010