Quantitative CT imaging of the spatio-temporal distribution patterns of vasa vasorum in aortas of apoE-/-/LDL-/- double knockout mice

M. Kampschulte, A. Brinkmann, P. Stieger, D. G. Sedding, C. Dierkes, R. M. Bohle, G. Krombach, Erik L. Ritman, A. C. Langheinrich

Research output: Contribution to journalArticle

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Abstract

Objective: To investigate the distribution of vasa vasorum (VV) relative to advanced atherosclerotic lesions (calcified, fibrotic or hemorrhaged) along the aortic wall of apoE-/-/LDL-/- mice at the age of 25 and 80 weeks using high-resolution nano-CT. Methods: Aortas from male apoE-/-/LDL-/- mice at the age of 25 weeks (n=4) and 80 weeks (n=7) were infused in situ with contrast agent and harvested for scanning with nano-CT. The spatial distribution of vasa vasorum [number and area/cross-section (mm2)] was compared to aortic luminal cross-sectional area and plaque cross-sectional area in the ascending aorta, aortic arch and descending aorta. Results were complemented with co-localized histology. Results: The number and total luminal cross-sectional area of VV showed a significant decrease in the ascending aorta and aortic arch from 25 to 80 weeks but not in the descending aorta. The number and cross-sectional area of VV showed significant local differences depending on whether it was near a fibrotic, and hemorrhaged or calcified plaque in animals at the age of 80 weeks. Area of VV progressively increased along the aorta from least in the ascending aorta < aortic arch < descending aorta in animals at the age of 80 weeks and is inverse in animals aged 25 weeks. Conclusion: Atherosclerotic lesion type is correlated to the number and cross-sectional area of VV in different aortic segments in apoE-/-/LDL-/- mice. The chronological development of VV along the aorta proceeds distally from the ascending aorta and aortic arch.

Original languageEnglish (US)
Pages (from-to)444-450
Number of pages7
JournalAtherosclerosis
Volume212
Issue number2
DOIs
StatePublished - Oct 2010

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Vasa Vasorum
Apolipoproteins E
Thoracic Aorta
Knockout Mice
Aorta
oxidized low density lipoprotein
Contrast Media
Histology

Keywords

  • Atherosclerosis
  • Imaging
  • Micro-CT
  • Nano-CT
  • Vasa vasorum

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Kampschulte, M., Brinkmann, A., Stieger, P., Sedding, D. G., Dierkes, C., Bohle, R. M., ... Langheinrich, A. C. (2010). Quantitative CT imaging of the spatio-temporal distribution patterns of vasa vasorum in aortas of apoE-/-/LDL-/- double knockout mice. Atherosclerosis, 212(2), 444-450. https://doi.org/10.1016/j.atherosclerosis.2010.07.010

Quantitative CT imaging of the spatio-temporal distribution patterns of vasa vasorum in aortas of apoE-/-/LDL-/- double knockout mice. / Kampschulte, M.; Brinkmann, A.; Stieger, P.; Sedding, D. G.; Dierkes, C.; Bohle, R. M.; Krombach, G.; Ritman, Erik L.; Langheinrich, A. C.

In: Atherosclerosis, Vol. 212, No. 2, 10.2010, p. 444-450.

Research output: Contribution to journalArticle

Kampschulte, M, Brinkmann, A, Stieger, P, Sedding, DG, Dierkes, C, Bohle, RM, Krombach, G, Ritman, EL & Langheinrich, AC 2010, 'Quantitative CT imaging of the spatio-temporal distribution patterns of vasa vasorum in aortas of apoE-/-/LDL-/- double knockout mice', Atherosclerosis, vol. 212, no. 2, pp. 444-450. https://doi.org/10.1016/j.atherosclerosis.2010.07.010
Kampschulte, M. ; Brinkmann, A. ; Stieger, P. ; Sedding, D. G. ; Dierkes, C. ; Bohle, R. M. ; Krombach, G. ; Ritman, Erik L. ; Langheinrich, A. C. / Quantitative CT imaging of the spatio-temporal distribution patterns of vasa vasorum in aortas of apoE-/-/LDL-/- double knockout mice. In: Atherosclerosis. 2010 ; Vol. 212, No. 2. pp. 444-450.
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abstract = "Objective: To investigate the distribution of vasa vasorum (VV) relative to advanced atherosclerotic lesions (calcified, fibrotic or hemorrhaged) along the aortic wall of apoE-/-/LDL-/- mice at the age of 25 and 80 weeks using high-resolution nano-CT. Methods: Aortas from male apoE-/-/LDL-/- mice at the age of 25 weeks (n=4) and 80 weeks (n=7) were infused in situ with contrast agent and harvested for scanning with nano-CT. The spatial distribution of vasa vasorum [number and area/cross-section (mm2)] was compared to aortic luminal cross-sectional area and plaque cross-sectional area in the ascending aorta, aortic arch and descending aorta. Results were complemented with co-localized histology. Results: The number and total luminal cross-sectional area of VV showed a significant decrease in the ascending aorta and aortic arch from 25 to 80 weeks but not in the descending aorta. The number and cross-sectional area of VV showed significant local differences depending on whether it was near a fibrotic, and hemorrhaged or calcified plaque in animals at the age of 80 weeks. Area of VV progressively increased along the aorta from least in the ascending aorta < aortic arch < descending aorta in animals at the age of 80 weeks and is inverse in animals aged 25 weeks. Conclusion: Atherosclerotic lesion type is correlated to the number and cross-sectional area of VV in different aortic segments in apoE-/-/LDL-/- mice. The chronological development of VV along the aorta proceeds distally from the ascending aorta and aortic arch.",
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AU - Kampschulte, M.

AU - Brinkmann, A.

AU - Stieger, P.

AU - Sedding, D. G.

AU - Dierkes, C.

AU - Bohle, R. M.

AU - Krombach, G.

AU - Ritman, Erik L.

AU - Langheinrich, A. C.

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N2 - Objective: To investigate the distribution of vasa vasorum (VV) relative to advanced atherosclerotic lesions (calcified, fibrotic or hemorrhaged) along the aortic wall of apoE-/-/LDL-/- mice at the age of 25 and 80 weeks using high-resolution nano-CT. Methods: Aortas from male apoE-/-/LDL-/- mice at the age of 25 weeks (n=4) and 80 weeks (n=7) were infused in situ with contrast agent and harvested for scanning with nano-CT. The spatial distribution of vasa vasorum [number and area/cross-section (mm2)] was compared to aortic luminal cross-sectional area and plaque cross-sectional area in the ascending aorta, aortic arch and descending aorta. Results were complemented with co-localized histology. Results: The number and total luminal cross-sectional area of VV showed a significant decrease in the ascending aorta and aortic arch from 25 to 80 weeks but not in the descending aorta. The number and cross-sectional area of VV showed significant local differences depending on whether it was near a fibrotic, and hemorrhaged or calcified plaque in animals at the age of 80 weeks. Area of VV progressively increased along the aorta from least in the ascending aorta < aortic arch < descending aorta in animals at the age of 80 weeks and is inverse in animals aged 25 weeks. Conclusion: Atherosclerotic lesion type is correlated to the number and cross-sectional area of VV in different aortic segments in apoE-/-/LDL-/- mice. The chronological development of VV along the aorta proceeds distally from the ascending aorta and aortic arch.

AB - Objective: To investigate the distribution of vasa vasorum (VV) relative to advanced atherosclerotic lesions (calcified, fibrotic or hemorrhaged) along the aortic wall of apoE-/-/LDL-/- mice at the age of 25 and 80 weeks using high-resolution nano-CT. Methods: Aortas from male apoE-/-/LDL-/- mice at the age of 25 weeks (n=4) and 80 weeks (n=7) were infused in situ with contrast agent and harvested for scanning with nano-CT. The spatial distribution of vasa vasorum [number and area/cross-section (mm2)] was compared to aortic luminal cross-sectional area and plaque cross-sectional area in the ascending aorta, aortic arch and descending aorta. Results were complemented with co-localized histology. Results: The number and total luminal cross-sectional area of VV showed a significant decrease in the ascending aorta and aortic arch from 25 to 80 weeks but not in the descending aorta. The number and cross-sectional area of VV showed significant local differences depending on whether it was near a fibrotic, and hemorrhaged or calcified plaque in animals at the age of 80 weeks. Area of VV progressively increased along the aorta from least in the ascending aorta < aortic arch < descending aorta in animals at the age of 80 weeks and is inverse in animals aged 25 weeks. Conclusion: Atherosclerotic lesion type is correlated to the number and cross-sectional area of VV in different aortic segments in apoE-/-/LDL-/- mice. The chronological development of VV along the aorta proceeds distally from the ascending aorta and aortic arch.

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