Quantitative analysis of three-dimensional fibrillar collagen microstructure within the normal, aged and glaucomatous human optic nerve head

H. J. Jones, M. J. Girard, N. White, Michael P Fautsch, J. E. Morgan, C. R. Ethier, J. Albon

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The aim of this study was to quantify connective tissue fibre orientation and alignment in young, old and glaucomatous human optic nerve heads (ONH) to understand ONH microstructure and predisposition to glaucomatous optic neuropathy. Transverse (seven healthy, three glaucomatous) and longitudinal (14 healthy) human ONH cryosections were imaged by both second harmonic generation microscopy and small angle light scattering (SALS) in order to quantify preferred fibre orientation (PFO) and degree of fibre alignment (DOFA). DOFA was highest within the peripapillary sclera (ppsclera), with relatively low values in the lamina cribrosa (LC). Elderly ppsclera DOFA was higher than that in young ppsclera (p< 0.00007), and generally higher than in glaucoma ppsclera. In all LCs, a majority of fibres had preferential orientation horizontally across the nasal-temporal axis. In all glaucomatous LCs, PFO was significantly different from controls in a minimum of seven out of 12 LC regions (p < 0.05). Additionally, higher fibre alignment was observed in the glaucomatous inferior-temporal LC (p < 0.017). The differences between young and elderly ONH fibre alignment within regions suggest that age-related microstructural changes occur within the structure. The additional differences in fibre alignment observed within the glaucomatous LC may reflect an inherent susceptibility to glaucomatous optic neuropathy, or may be a consequence of ONH remodelling and/or collapse.

Original languageEnglish (US)
Article number20150066
JournalJournal of the Royal Society Interface
Volume12
Issue number106
DOIs
StatePublished - May 6 2015

Fingerprint

Fibrillar Collagens
Optic Disk
Sclera
Collagen
Optics
Microstructure
Fibers
Chemical analysis
Optic Nerve Diseases
Fiber reinforced materials
Small Angle Scattering
Nerve Fibers
Nose
Glaucoma
Connective Tissue
Microscopy
Harmonic generation
Light scattering
Light
Microscopic examination

Keywords

  • Connective tissue
  • Fibre orientation and alignment
  • Glaucoma
  • Optic nerve head
  • Second harmonic generation
  • Small angle light scattering

ASJC Scopus subject areas

  • Biophysics
  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials
  • Biochemistry

Cite this

Quantitative analysis of three-dimensional fibrillar collagen microstructure within the normal, aged and glaucomatous human optic nerve head. / Jones, H. J.; Girard, M. J.; White, N.; Fautsch, Michael P; Morgan, J. E.; Ethier, C. R.; Albon, J.

In: Journal of the Royal Society Interface, Vol. 12, No. 106, 20150066, 06.05.2015.

Research output: Contribution to journalArticle

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