Quantitative analysis of clonal bone marrow CD19+ B cells: Use of B cell lineage trees to delineate their role in the pathogenesis of light chain amyloidosis

Michelle K. Manske, Neta S. Zuckerman, Michael M. Timm, Stephanie Maiden, Hanna Edelman, Gitit Shahaf, Michal Barak, Angela Dispenzieri, Morie A. Gertz, Ramit Mehr, Roshini S. Abraham

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Light chain amyloidosis (AL) is a bone marrow (BM) plasma cell neoplasia with systemic deposition of Ig light chain amyloid fibrils. Here, we report the identification of clonal CD19 B cells in the BM and the use of a novel mathematical algorithm to generate B cell lineage trees of the clonal CD19 B cells and CD138 plasma cells from the BM of AL patients to delineate the relationship between these two clonal populations. The CD19+ clonal B cells in the BM of AL patients related to the clonal plasma cells represent a pre-plasma cell precursor population. The B cell lineage trees from AL patients also show significant differences in clonal diversification and antigenic selection compared to clones from normal, healthy controls. These data provide a robust example of the use of graphical quantification methods in delineating the role of neoplastic precursors in the pathogenesis of hematopoietic malignancies.

Original languageEnglish (US)
Pages (from-to)106-120
Number of pages15
JournalClinical Immunology
Volume120
Issue number1
DOIs
StatePublished - Jul 2006

Keywords

  • B cells
  • Clone
  • Human
  • Immunoglobulin light chain amyloidosis
  • Monoclonal gammopathy
  • Plasma cell dyscrasia

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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