Abstract
Parathyroid hormone (PTH), an 84 amino acid peptide hormone, is an important regulator of calcium homeostasis. Quantitation of PTH in serum is useful for the diagnosis of primary hyperparathyroidism, hypoparathyroidism, and for monitoring osteodystrophy in patients with renal failure. The biological activity of PTH arises from binding of PTH (N terminus) to its target receptor (D’Amour et al., Kidney Int 68: 998–1007, 2005). Several C-terminal and N-terminal fragments circulate in normal subjects. Recent studies have demonstrated that accurate quantitation of PTH fragments may be of clinical value. In this chapter a mass spectrometry based method for quantitation of PTH(1–84) is described. This method involves immunoaffinity capture of PTH followed by trypsinization and quantitation of PTH-specific tryptic peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The N-terminal tryptic peptide, PTH(1–13) as surrogate of 1–84 PTH, is used for quantitation.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 211-217 |
| Number of pages | 7 |
| Volume | 1378 |
| DOIs | |
| State | Published - 2016 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1378 |
| ISSN (Print) | 10643745 |
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Keywords
- Immunocapture LC-MS/MS
- LC-MS/MS
- Parathyroid hormone quantitation
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Quantitation of parathyroid hormone in serum or plasma by liquid chromatography-tandem mass spectrometry. / Ketha, Hemamalini; Singh, Ravinder Jit.
Methods in Molecular Biology. Vol. 1378 Humana Press Inc., 2016. p. 211-217 (Methods in Molecular Biology; Vol. 1378).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Quantitation of parathyroid hormone in serum or plasma by liquid chromatography-tandem mass spectrometry
AU - Ketha, Hemamalini
AU - Singh, Ravinder Jit
PY - 2016
Y1 - 2016
N2 - Parathyroid hormone (PTH), an 84 amino acid peptide hormone, is an important regulator of calcium homeostasis. Quantitation of PTH in serum is useful for the diagnosis of primary hyperparathyroidism, hypoparathyroidism, and for monitoring osteodystrophy in patients with renal failure. The biological activity of PTH arises from binding of PTH (N terminus) to its target receptor (D’Amour et al., Kidney Int 68: 998–1007, 2005). Several C-terminal and N-terminal fragments circulate in normal subjects. Recent studies have demonstrated that accurate quantitation of PTH fragments may be of clinical value. In this chapter a mass spectrometry based method for quantitation of PTH(1–84) is described. This method involves immunoaffinity capture of PTH followed by trypsinization and quantitation of PTH-specific tryptic peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The N-terminal tryptic peptide, PTH(1–13) as surrogate of 1–84 PTH, is used for quantitation.
AB - Parathyroid hormone (PTH), an 84 amino acid peptide hormone, is an important regulator of calcium homeostasis. Quantitation of PTH in serum is useful for the diagnosis of primary hyperparathyroidism, hypoparathyroidism, and for monitoring osteodystrophy in patients with renal failure. The biological activity of PTH arises from binding of PTH (N terminus) to its target receptor (D’Amour et al., Kidney Int 68: 998–1007, 2005). Several C-terminal and N-terminal fragments circulate in normal subjects. Recent studies have demonstrated that accurate quantitation of PTH fragments may be of clinical value. In this chapter a mass spectrometry based method for quantitation of PTH(1–84) is described. This method involves immunoaffinity capture of PTH followed by trypsinization and quantitation of PTH-specific tryptic peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The N-terminal tryptic peptide, PTH(1–13) as surrogate of 1–84 PTH, is used for quantitation.
KW - Immunocapture LC-MS/MS
KW - LC-MS/MS
KW - Parathyroid hormone quantitation
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UR - http://www.scopus.com/inward/citedby.url?scp=84948798237&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3182-8_22
DO - 10.1007/978-1-4939-3182-8_22
M3 - Chapter
C2 - 26602132
AN - SCOPUS:84948798237
VL - 1378
T3 - Methods in Molecular Biology
SP - 211
EP - 217
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -