Quantitation of parathyroid hormone in serum or plasma by liquid chromatography-tandem mass spectrometry

Hemamalini Ketha, Ravinder Jit Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Parathyroid hormone (PTH), an 84 amino acid peptide hormone, is an important regulator of calcium homeostasis. Quantitation of PTH in serum is useful for the diagnosis of primary hyperparathyroidism, hypoparathyroidism, and for monitoring osteodystrophy in patients with renal failure. The biological activity of PTH arises from binding of PTH (N terminus) to its target receptor (D’Amour et al., Kidney Int 68: 998–1007, 2005). Several C-terminal and N-terminal fragments circulate in normal subjects. Recent studies have demonstrated that accurate quantitation of PTH fragments may be of clinical value. In this chapter a mass spectrometry based method for quantitation of PTH(1–84) is described. This method involves immunoaffinity capture of PTH followed by trypsinization and quantitation of PTH-specific tryptic peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The N-terminal tryptic peptide, PTH(1–13) as surrogate of 1–84 PTH, is used for quantitation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages211-217
Number of pages7
Volume1378
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1378
ISSN (Print)10643745

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Keywords

  • Immunocapture LC-MS/MS
  • LC-MS/MS
  • Parathyroid hormone quantitation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ketha, H., & Singh, R. J. (2016). Quantitation of parathyroid hormone in serum or plasma by liquid chromatography-tandem mass spectrometry. In Methods in Molecular Biology (Vol. 1378, pp. 211-217). (Methods in Molecular Biology; Vol. 1378). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3182-8_22