Quantitation of insulin-like growth factor 1 in serum by liquid chromatography high resolution accurate-mass mass spectrometry

Hemamalini Ketha, Ravinder Jit Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Insulin-like growth factor 1 (IGF-1) is a 70 amino acid peptide hormone which acts as the principal mediator of the effects of growth hormone (GH). Due to a wide variability in circulating concentration of GH, IGF-1 quantitation is the first step in the diagnosis of GH excess or deficiency. Majority (>95%) of IGF-1 circulates as a ternary complex along with its principle binding protein insulin-like growth factor 1 binding protein 3 (IGFBP-3) and acid labile subunit. The assay design approach for IGF-1 quantitation has to include a step to dissociate IGF-1 from its ternary complex. Several commercial assays employ a buffer containing acidified ethanol to achieve this. Despite several modifications, commercially available immunoassays have been shown to have challenges with interference from IGFBP-3. Additionally, inter-method comparison between IGF-1 immunoassays has been shown to be suboptimal. Mass spectrometry has been utilized for quantitation of IGF-1. In this chapter a liquid chromatography high resolution accurate-mass mass spectrometry (LC-HRAMS) based method for IGF-1 quantitation has been described.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages131-137
Number of pages7
Volume1378
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1378
ISSN (Print)10643745

Fingerprint

Somatomedins
Liquid Chromatography
Mass Spectrometry
Serum
Insulin-Like Growth Factor Binding Protein 3
Immunoassay
Growth Hormone
Peptide Hormones
Carrier Proteins
Buffers
Ethanol
Amino Acids
Acids

Keywords

  • High resolution mass spectrometry
  • Insulin -like growth factor-1 quantitation
  • Protein quantitation using mass spectrometry

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ketha, H., & Singh, R. J. (2016). Quantitation of insulin-like growth factor 1 in serum by liquid chromatography high resolution accurate-mass mass spectrometry. In Methods in Molecular Biology (Vol. 1378, pp. 131-137). (Methods in Molecular Biology; Vol. 1378). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3182-8_15

Quantitation of insulin-like growth factor 1 in serum by liquid chromatography high resolution accurate-mass mass spectrometry. / Ketha, Hemamalini; Singh, Ravinder Jit.

Methods in Molecular Biology. Vol. 1378 Humana Press Inc., 2016. p. 131-137 (Methods in Molecular Biology; Vol. 1378).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ketha, H & Singh, RJ 2016, Quantitation of insulin-like growth factor 1 in serum by liquid chromatography high resolution accurate-mass mass spectrometry. in Methods in Molecular Biology. vol. 1378, Methods in Molecular Biology, vol. 1378, Humana Press Inc., pp. 131-137. https://doi.org/10.1007/978-1-4939-3182-8_15
Ketha H, Singh RJ. Quantitation of insulin-like growth factor 1 in serum by liquid chromatography high resolution accurate-mass mass spectrometry. In Methods in Molecular Biology. Vol. 1378. Humana Press Inc. 2016. p. 131-137. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3182-8_15
Ketha, Hemamalini ; Singh, Ravinder Jit. / Quantitation of insulin-like growth factor 1 in serum by liquid chromatography high resolution accurate-mass mass spectrometry. Methods in Molecular Biology. Vol. 1378 Humana Press Inc., 2016. pp. 131-137 (Methods in Molecular Biology).
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