Quantitation of insulin-like growth factor 1 in serum by liquid chromatography high resolution accurate-mass mass spectrometry

Hemamalini Ketha, Ravinder Jit Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Insulin-like growth factor 1 (IGF-1) is a 70 amino acid peptide hormone which acts as the principal mediator of the effects of growth hormone (GH). Due to a wide variability in circulating concentration of GH, IGF-1 quantitation is the first step in the diagnosis of GH excess or deficiency. Majority (>95%) of IGF-1 circulates as a ternary complex along with its principle binding protein insulin-like growth factor 1 binding protein 3 (IGFBP-3) and acid labile subunit. The assay design approach for IGF-1 quantitation has to include a step to dissociate IGF-1 from its ternary complex. Several commercial assays employ a buffer containing acidified ethanol to achieve this. Despite several modifications, commercially available immunoassays have been shown to have challenges with interference from IGFBP-3. Additionally, inter-method comparison between IGF-1 immunoassays has been shown to be suboptimal. Mass spectrometry has been utilized for quantitation of IGF-1. In this chapter a liquid chromatography high resolution accurate-mass mass spectrometry (LC-HRAMS) based method for IGF-1 quantitation has been described.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages131-137
Number of pages7
Volume1378
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1378
ISSN (Print)10643745

Keywords

  • High resolution mass spectrometry
  • Insulin -like growth factor-1 quantitation
  • Protein quantitation using mass spectrometry

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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