Quantitation of albumin in urine by liquid chromatography tandem mass spectrometry

Hemamalini Ketha, Ravinder Jit Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Urinary excretion of human serum albumin (HSA), a 6.65 kDa monomeric protein, is a sensitive marker of renal damage associated with many diseases including diabetes mellitus. Albumin is synthesized by the liver and functions as a transport protein for fat-soluble hormones and drugs and for maintaining plasma colloid osmotic pressure and pH. Albumin is not filtered at the glomerulus and its presence in the urine at concentration above 30 mg/day is suggestive of glomerular damage. Early diagnosis of microalbuminuria (30–300 mg/24 h urine albumin excretion or 30–300 mg/g creatinine in random collections) has prognostic value for monitoring disease progression and early clinical management of diabetic nephropathy in prediabetic patients. Current methods for quantitation of urine albumin are based on immunoassays or size exclusion high-performance liquid chromatography coupled with UV detection (SEC-HPLC-UV). Studies have demonstrated discordance between the existing methods. It has been suggested that while immunoassays underestimate albumin in urine, SEC-HPLC-UV method overestimates albumin as it cannot separate co-eluting interferences. This chapter describes a liquid chromatography tandem mass spectrometry LC-MS/MS candidate reference method for albumin quantitation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages31-36
Number of pages6
Volume1378
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1378
ISSN (Print)10643745

Fingerprint

Tandem Mass Spectrometry
Liquid Chromatography
Albumins
Urine
Immunoassay
High Pressure Liquid Chromatography
Osmotic Pressure
Diabetic Nephropathies
Colloids
Serum Albumin
Disease Progression
Early Diagnosis
Creatinine
Diabetes Mellitus
Carrier Proteins
Fats
Hormones
Kidney
Liver
Pharmaceutical Preparations

Keywords

  • Albumin
  • LC-MS/MS
  • Microalbumin
  • Microalbuminuria

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ketha, H., & Singh, R. J. (2016). Quantitation of albumin in urine by liquid chromatography tandem mass spectrometry. In Methods in Molecular Biology (Vol. 1378, pp. 31-36). (Methods in Molecular Biology; Vol. 1378). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3182-8_4

Quantitation of albumin in urine by liquid chromatography tandem mass spectrometry. / Ketha, Hemamalini; Singh, Ravinder Jit.

Methods in Molecular Biology. Vol. 1378 Humana Press Inc., 2016. p. 31-36 (Methods in Molecular Biology; Vol. 1378).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ketha, H & Singh, RJ 2016, Quantitation of albumin in urine by liquid chromatography tandem mass spectrometry. in Methods in Molecular Biology. vol. 1378, Methods in Molecular Biology, vol. 1378, Humana Press Inc., pp. 31-36. https://doi.org/10.1007/978-1-4939-3182-8_4
Ketha H, Singh RJ. Quantitation of albumin in urine by liquid chromatography tandem mass spectrometry. In Methods in Molecular Biology. Vol. 1378. Humana Press Inc. 2016. p. 31-36. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3182-8_4
Ketha, Hemamalini ; Singh, Ravinder Jit. / Quantitation of albumin in urine by liquid chromatography tandem mass spectrometry. Methods in Molecular Biology. Vol. 1378 Humana Press Inc., 2016. pp. 31-36 (Methods in Molecular Biology).
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