Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS).

Ravinder J. Singh

doi:10.1007/978-1-60761-459-3_50

Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS).

Ravinder J. Singh

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.

Original language	English (US)
Pages (from-to)	509-517
Number of pages	9
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	603
DOIs	https://doi.org/10.1007/978-1-60761-459-3_50
State	Published - 2010

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-60761-459-3_50

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abstract = "25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.",

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AB - 25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.

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Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS).

Abstract

ASJC Scopus subject areas

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