Abstract
25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 509-517 |
| Number of pages | 9 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 603 |
| DOIs | |
| State | Published - 2010 |
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ASJC Scopus subject areas
- Medicine(all)
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Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS). / Singh, Ravinder Jit.
In: Methods in molecular biology (Clifton, N.J.), Vol. 603, 2010, p. 509-517.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS).
AU - Singh, Ravinder Jit
PY - 2010
Y1 - 2010
N2 - 25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.
AB - 25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.
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UR - http://www.scopus.com/inward/citedby.url?scp=77950344862&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-459-3_50
DO - 10.1007/978-1-60761-459-3_50
M3 - Article
C2 - 20077103
AN - SCOPUS:77950344862
VL - 603
SP - 509
EP - 517
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -