Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS).

Ravinder J. Singh

doi:10.1007/978-1-60761-459-3_50

Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS).

Ravinder J. Singh

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.

Original language	English (US)
Pages (from-to)	509-517
Number of pages	9
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	603
DOIs	https://doi.org/10.1007/978-1-60761-459-3_50
State	Published - 2010

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-60761-459-3_50

Fingerprint Dive into the research topics of 'Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS).'. Together they form a unique fingerprint.

Cite this

@article{cb11fa382ab849a8bc7826a51b135d74,

title = "Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS).",

abstract = "25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.",

author = "Singh, {Ravinder J.}",

year = "2010",

doi = "10.1007/978-1-60761-459-3_50",

language = "English (US)",

volume = "603",

pages = "509--517",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS).

AU - Singh, Ravinder J.

PY - 2010

Y1 - 2010

N2 - 25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.

AB - 25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.

UR - http://www.scopus.com/inward/record.url?scp=77950344862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950344862&partnerID=8YFLogxK

U2 - 10.1007/978-1-60761-459-3_50

DO - 10.1007/978-1-60761-459-3_50

M3 - Article

C2 - 20077103

AN - SCOPUS:77950344862

VL - 603

SP - 509

EP - 517

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -