Quantification of three-dimensional dynamics of intercellular geometry under mechanical loading using a weighted directional adaptive-threshold method

Nikola Kojic, Austin Huang, Chung Euiheon, Daniel Tschumperlin, Peter T.C. So

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Capturing and quantifying dynamic changes in three-dimensional cellular geometries on fast time scales is a challenge because of mechanical limitations of imaging systems as well as of the inherent tradeoffs between temporal resolution and image quality. We have combined a custom high-speed two-photon microscopy approach with a novel image segmentation method, the weighted directional adaptive-threshold (WDAT), to quantify the dimensions of intercellular spaces of cells under compressive stress on timescales previously inaccessible. The adaptation of a high-speed two-photon microscope addressed the need to capture events occurring on short timescales, while the WDAT method was developed to address artifacts of standard intensity-based analysis methods when applied to this system. Our novel approach is demonstrated by the enhanced temporal analysis of the three-dimensional cellular and extracellular deformations that accompany compressive loading of airway epithelial cells.

Original languageEnglish (US)
Pages (from-to)12403-12414
Number of pages12
JournalOptics Express
Volume16
Issue number16
DOIs
StatePublished - Aug 4 2008

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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