Quantification of RNA content in reconstituted ebola virus nucleocapsids by immunoprecipitation

Logan Banadyga, Hideki Ebihara

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Immunoprecipitations are commonly used to isolate proteins or protein complexes and assess protein-protein interactions; however, they can also be used to assess protein-RNA complexes. Here we describe an adapted RNA immunoprecipitation technique that permits the quantification of RNA content in Ebola virus nucleocapsids that have been reconstituted in vitro by transient transfection.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages93-107
Number of pages15
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1628
ISSN (Print)1064-3745

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Keywords

  • Ebola virus
  • Immunoprecipitation
  • Nucleocapsid
  • Nucleoprotein
  • qRT-PCR
  • RNA
  • VP24
  • VP35

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Banadyga, L., & Ebihara, H. (2017). Quantification of RNA content in reconstituted ebola virus nucleocapsids by immunoprecipitation. In Methods in Molecular Biology (pp. 93-107). (Methods in Molecular Biology; Vol. 1628). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7116-9_7